J Korean Med Sci.  2015 Jul;30(7):866-870. 10.3346/jkms.2015.30.7.866.

Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi

Affiliations
  • 1Translation Research Center, Inha University School of Medicine, Incheon, Korea.
  • 2Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea. ljinsoo@inha.ac.kr
  • 3Department of Microbiology, Inha University School of Medicine, Incheon, Korea.

Abstract

Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.

Keyword

Orientia tsutsugamushi; Scrub Typhus; Outer Membrane Vesicles; Antigens

MeSH Terms

Antibodies, Monoclonal/*immunology
Antigens, Bacterial/*immunology
Antigens, Surface/*immunology
Cell Line
Cell Membrane/immunology
Humans
Microscopy, Electron
Orientia tsutsugamushi/*immunology/metabolism
Scrub Typhus/diagnosis/microbiology
Secretory Vesicles/*immunology
Antibodies, Monoclonal
Antigens, Bacterial
Antigens, Surface
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