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Bordetella bronchiseptica antigen enhances the production of Mycoplasma hyopneumoniae antigen-specific immunoglobulin G in mice

Yim SH, Hahn TW, Joo HG

We previously demonstrated that Bordetella (B.) bronchiseptica antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma (M.) hyopneumoniae Ag showed low effects. The focus of...
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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but...
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Clinical Evaluation of Rapid Diagnostic Test Kit Using the Polysaccharide as a Genus-Specific Diagnostic Antigen for Leptospirosis in Korea, Bulgaria, and Argentina

Lee JW, Park S, Kim SH, Christova , Jacob P, Vanasco NB, Kang YM, Woo YJ, Kim MS, Kim YJ, Cho MK, Kim YW

Leptospirosis, a zoonotic disease that is caused by many serovars which are more than 200 in the world, is an emerging worldwide disease. Accurate and rapid diagnostic tests for leptospirosis...
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Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi

Lee SM, Kwon HY, Im JH, Baek JH, Kang JS, Lee JS

Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to...
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Evaluation of adjuvant effects of fucoidan for improving vaccine efficacy

Kim SY, Joo HG

Fucoidan is a sulfated polysaccharide derived from brown seaweed, including Fucus vesiculosus. This compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic...
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Antigen targeting to M cells for enhancing the efficacy of mucosal vaccines

Kim SH, Jang YS

Vaccination is one of the most successful applications of immunology and for a long time has depended on parenteral administration protocols. However, recent studies have pointed to the promise of...
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Regulatory T-cell vaccination independent of auto-antigen

Pascual DW, Yang X, Holderness K, Jun S, Maddaloni , Kochetkova I

To date, efforts to treat autoimmune diseases have primarily focused on the disease symptoms rather than on the cause of the disease. In large part, this is attributed to not...
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Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. paratuberculosis

Shin SJ, Yoo HS, McDonough , Chang YF

  • KMID: 1120066
  • J Vet Sci.
  • 2004 Jun;5(2):111-117.
Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of...
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Efficacy of Purified Vi Polysaccharide Typhoid Vaccine

Park JH, Hong JJ, Choi ES, Lee JW, Park JH

  • KMID: 1095363
  • J Vet Sci.
  • 2002 Jun;3(2):67-70.
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2)...
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Reaction of the sera of the Korean children free from Hib invasive diseases against H. influenzae type B capsular polysaccharide antigen

Kim KH, Kim DS, Park MS, Kim KT, Kim HS, Kim KH, Kwon OH

The purpose of our experiment is to examine the level of anti-Haemophilus influenza polysaccharide antibody titer in the Korean population. Using ELISA, the level of Hib-PS antibodies in 384 infants...
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