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Bordetella bronchiseptica antigen enhances the production of Mycoplasma hyopneumoniae antigen-specific immunoglobulin G in mice

Yim SH, Hahn TW, Joo HG

We previously demonstrated that Bordetella (B.) bronchiseptica antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma (M.) hyopneumoniae Ag showed low effects. The focus of...
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Roles of intestinal epithelial cells in the maintenance of gut homeostasis

Okumura R, Takeda K

The intestine is a unique organ inhabited by a tremendous number of microorganisms. Intestinal epithelial cells greatly contribute to the maintenance of the symbiotic relationship between gut microbiota and the...
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Correlations between the CagA Antigen and Serum Levels of Anti-Helicobacter pylori IgG and IgA in Children

Seo JH, Lim CW, Park JS, Yeom JS, Lim JY, Jun JS, Woo HO, Youn HS, Baik SC, Lee WK, Cho MJ, Rhee KH

We tested correlations between anti-Helicobacter pylori IgG and IgA levels and the urease test, anti-CagA protein antibody, degree of gastritis, and age. In total, 509 children (0-15 years) were enrolled....
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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but...
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Clinical Evaluation of Rapid Diagnostic Test Kit Using the Polysaccharide as a Genus-Specific Diagnostic Antigen for Leptospirosis in Korea, Bulgaria, and Argentina

Lee JW, Park S, Kim SH, Christova , Jacob P, Vanasco NB, Kang YM, Woo YJ, Kim MS, Kim YJ, Cho MK, Kim YW

Leptospirosis, a zoonotic disease that is caused by many serovars which are more than 200 in the world, is an emerging worldwide disease. Accurate and rapid diagnostic tests for leptospirosis...
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RUNX3 Methylation, Loss of RUNX3 Expression and Clinicopathologic Findings according to Helicobacter pylori CagA in Gastric Carcinoma

Na YJ, Shim KN, Joo YH, Kim SE, Jung HK, Jung SA, Cho MS

BACKGROUND/AIMS: Helicobacter pylori cytotoxin-associated gene A (CagA) has been suggested to be involved in the inactivation of Runt-related transcription factor 3 (RUNX3), a known gastric carcinoma tumor suppressor gene. It...
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Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi

Lee SM, Kwon HY, Im JH, Baek JH, Kang JS, Lee JS

Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to...
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Evaluation of adjuvant effects of fucoidan for improving vaccine efficacy

Kim SY, Joo HG

Fucoidan is a sulfated polysaccharide derived from brown seaweed, including Fucus vesiculosus. This compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic...
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Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay

Je S, Yeo UC, Song T, Kim KC, Park SY, Kim MJ, Cho SN

The interferon-gamma (IFN-gamma) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the...
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Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

Alves RN, Rieck SE, Ueira-Vieira C, Labruna MB, Beletti ME

Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation...
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Development of a Quantitative Sandwich Enzyme-Linked Immunosorbent Assay for Detecting the MPT64 Antigen of Mycobacterium tuberculosis

Ji M, Cho B, Cho YS, Park SY, Cho SN, Jeon BY, Yoon BS

PURPOSE: Tuberculosis (TB) is a major infectious disease and is responsible for two million deaths annually. For the identification and quantitation of Mycobacterium tuberculosis (M. tuberculosis), a causative agent of...
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Antigen targeting to M cells for enhancing the efficacy of mucosal vaccines

Kim SH, Jang YS

Vaccination is one of the most successful applications of immunology and for a long time has depended on parenteral administration protocols. However, recent studies have pointed to the promise of...
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Regulatory T-cell vaccination independent of auto-antigen

Pascual DW, Yang X, Holderness K, Jun S, Maddaloni , Kochetkova I

To date, efforts to treat autoimmune diseases have primarily focused on the disease symptoms rather than on the cause of the disease. In large part, this is attributed to not...
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Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Rossi L, Di Giancamillo A, Reggi S, Domeneghini C, Baldi A, Sala V, Dell'Orto , Coddens A, Cox E, Fogher C

Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral...
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Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived from Orientia tsutsugamushi Serotypes

Kim YJ, Yeo SJ, Park SJ, Woo YJ, Kim MW, Kim SH, Chang IA, Jeon SH, Park BJ, Song GJ, Lee MG, Kim IS, Kim YW

Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub...
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An Inverse Relationship between the Expression of the Gastric Tumor Suppressor RUNX3 and Infection with Helicobacter pylori in Gastric Epithelial Dysplasia

Chung WC, Jung SH, Joo KR, Kim MJ, Youn GJ, Kim Y, Lee JS, Lee H, Jung JH, Lee YK

  • KMID: 1712329
  • Gut Liver.
  • 2013 Nov;7(6):688-695.
BACKGROUND/AIMS: This study was performed to determine the association between RUNX3 expression and Helicobacter pylori infection in premalignant gastric lesions. METHODS: We examined 107 patients with gastric epithelial dysplasia who had...
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Persistence of Orientia tsutsugamushi in Humans

Chung MH, Lee JS, Baek JH, Kim M, Kang JS

We investigated the persistence of viable Orientia tsutsugamushi in patients who had recovered from scrub typhus. Blood specimens were available from six patients with scrub typhus who were at 1...
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Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

Joseph S, Thomas N, Thangapandian E, Singh VP, Verma R, Srivastava SK

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared...
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Relationship between Helicobacter pylori Virulence Genes and Clinical Outcomes in Saudi Patients

Marie MA

Helicobacter pylori has been strongly associated with gastritis, gastric and duodenal ulcers, and it is a risk factor for gastric cancer. Two major virulence factors of H. pylori have been...
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Three Cases of Anti-K and the KEL Gene Frequency in the Korean Population

Chang HE, Lee K, Hong YJ, Kim T, Song SH, Park KU, Song J, Han KS

  • KMID: 2300013
  • Korean J Blood Transfus.
  • 2011 Apr;22(1):31-37.
BACKGROUND: Anti-K is one of the most significant unexpected antibodies that cause hemolytic transfusion reactions. Individuals with anti-K have to be transfused with K antigen-negative red cells. Although Koreans rarely...
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