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Analysis of antigenic characteristics of Rickettsia tsutsugamushi Boryong strain and antigenic heterogeneity of Rickettsia tsutsugamushi using monoclonal antibodies

Park CS, Kim IC, Lee JB, Choi MS, Choi SB, Chang WH, Kim IS

Twenty-four monoclonal antibodies were produced by immunizing BALB/c mice with Rickettsia tsutsugamushi Boryong strain and used for the analysis of antigenic characteristics of R.tsutsugamushi Boryong strain and antigenic heterogeneity of...
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The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)

Davis WC, Khalid AM, Hamilton M, Ahn JS, Park YH, Cantor GH

  • KMID: 1103516
  • J Vet Sci.
  • 2001 Aug;2(2):103-109.
One of the major difficulties in studying the mechanisms of host defense in economically important species indigenous to Asia and the Middle East is the lack of monoclonal antibody (mAb)...
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Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Varshney B, Ponnanna N, Sarkar PA, Rehman P, Shah JH

Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies...
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Monoclonal antibody production and characterization for the measurement of plasma high density lipoprotein

Lee SH, Cho MH

We prepared two monoclonal antibodies-A-I30 and A-I4 to HDL apo A-I-with the ultimate goals of expressing and overproducing the valuable immunodiagnostic single chain Fv by phage display libraries in E,...
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Characterization of Antigenic Sites on the Rinderpest Virus N Protein Uusing Monoclonal Antibodies

Choi KS, Nah JJ, Ko YJ, Choi CU, Kim JH, Kang SY, Joo YS

  • KMID: 1099822
  • J Vet Sci.
  • 2003 Apr;4(1):57-65.
The N protein of the rinderpest virus (RPV) was analyzed topologically and antigenically by using anti-N monoclonal antibodies (Mabs). Ten Mabs were raised against the N protein of the RPV....
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Anti-IL-4 antibody inhibits antigen specific IgE response but fails to prevent chicken gamma globulin-induced active systemic anaphylaxis: evidence for the involvement of IgG antibodies

Lee HK, Lee HH, Park YM, Park HJ, Lee JH, Ha TY

It has recently been reported that interleukin-4 (IL-4) is required for the production of IgE, and anti-IL-4 monoclonal antibody (mAb) inhibits in vivo IgE responses. These suggest that blocking of...
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Immunoradiometric assay of human proinsulin and partially processed proinsulin with use of monoclonal antibody and streptavidin-biotin labeling

Kim JQ, Cho HI, Kim SI, Lee HK, Hales CN

The sensitive and specific immunoradiometric assay is described for human proinsulin and its intermediate peptides (65-66 split and 32-33 split proinsulin). We developed a monoclonal antibody-based two-site immunoradiometric assay with...
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A monoclonal antibody to common acute lymphoblastic leukemia antigen (CALLA) and its expression on several human tumor cell lines

Park WS, Hwang TS, Kim TJ, Park CS, Bae YM, Park SH, Lee SK

We describe a newly-made murine monoclonal antibody to the common acute lymphoblastic leukemia antigen (CALLA), named SHB-10. The antigen detected by SHB-10 has a molecular weight of about 105...
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In vitro immunohistochemical localization of S-phase cells by a monoclonal antibody to bromodeoxyuridine

Lee SS, Lee TS, Park JS, Cho EK, Chung CH

Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In vitro immunohistochemical application of...
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Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi

Lee SM, Kwon HY, Im JH, Baek JH, Kang JS, Lee JS

Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to...
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Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes

Han ET, Yang HJ, Park YJ, Park JH, Chai JY

  • KMID: 1792170
  • Korean J Parasitol.
  • 2014 Apr;52(2):201-204.
This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity...
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Characterization and epitope mapping of two monoclonal antibodies against human CD99

Gil MC, Lee MH, Seo JI, Choi YL, Kim MK, Jung KC, Park SH, Kim TJ

  • KMID: 1121094
  • Exp Mol Med.
  • 2002 Dec;34(6):411-418.
CD99 plays an critical role in the diapedesis of monocytes, T cell differentiation, and the transport of MHC molecules. Engagement of CD99 by agonistic monoclonal antibodies has been reported...
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Immunohistochemical analysis of p53 protein expression in benign and malignant skin tumors using a panel of anti-p53 antibodies

Ro YS, Kim JH

The expression of p53 in a variety of benign and malignant skin lesions has been first assessed in frozen sections and then compared with the results obtained in corresponding paraffin-embedded...
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C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

Tozaki K, Kimura J, Yasuda M, Ryu N, Nasu T, Pernthaner A, Hein WR

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a...
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Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

Davis WC, Hamilton M

Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for...
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Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody

Kim MJ, Kim MK, Kang JS

Orientia tsutsugamushi causes scrub typhus, which is endemic in many countries in the Asia-Pacific region including Korea. Recent emergence of doxycycline-resistant strains from Thailand has underlined the importance of the...
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New monoclonal antibody-based test for Helicobacter pylori urease in gastric tissue

Kim DH, Kim HD, Park H, Choi S, Beom JW, Kim WJ, Park CK, Lee YJ, Park JY, Kim HR, Park C, Joo YE, Jung YD

BACKGROUND/AIMS: To evaluate a new monoclonal antibody for Helicobacter pylori urease in gastric tissue. METHODS: A total of 107 volunteers were enrolled. All subjects underwent a 13C-urea breath test and esophagogastroduodenoscopy....
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Human glutamate dehydrogenase is immunologically distinct from other mammalian orthologues

Jang SH, Kim AY, Bahn JH, Eum WS, Kim DW, Park J, Lee KS, Kang TC, Won MH, Kang JH, Kwon OS, Yoon HY, Lee EY, Cho SW, Choi SY

  • KMID: 1109312
  • Exp Mol Med.
  • 2003 Aug;35(4):249-256.
Five monoclonal antibodies (mAbs) that recognize human glutamate dehydrogenase (GDH) have been selected and designated as monoclonal antibodies hGDH60-6, hGDH60-8, hGDH63-10, hGDH63-11, and hGDH91-14. A total of five mAbs recognizing...
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Relationship between ganglioside expression and anti-cancer effects of the monoclonal antibody against epithelial cell adhesion molecule in colon cancer

Kwak DH, Ryu JS, Kim CH, Ko K, Ma JY, Hwang KA, Choo YK

The human colorectal carcinoma-associated GA733 antigen epithelial cell adhesion molecule (EpCAM) was initially described as a cell surface protein selectively expressed in some myeloid cancers. Gangliosides are sialic acid-containing glycosphingolipids...
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Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Cha SH, Kim SH, Bischoff K, Kim HJ, Son SW, Kang HG

A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to...
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