Exp Mol Med.
2015 Mar;47(3):e154. 10.1038/emm.2015.7.
Analyses of the TCR repertoire of MHC class II-restricted innate CD4+ T cells
- Affiliations
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- 1Graduate School of Immunology, Seoul National University College of Medicine, Seoul, Korea. pshoe@snu.ac.kr
- 2Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
- 3Department of Biomedical Science, Seoul National University College of Medicine, Seoul, Korea.
- 4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
- 5Department of Molecular and Cellular Biology, Sungkyunkwan University, School of Medicine, Gyeonggi-do, Suwon, Korea.
- 6Transplantation Research Institute, Medical Research Center, Seoul National University, Seoul, Korea.
- KMID: 2155419
- DOI: http://doi.org/10.1038/emm.2015.7
Abstract
- Analysis of the T-cell receptor (TCR) repertoire of innate CD4+ T cells selected by major histocompatibility complex (MHC) class II-dependent thymocyte-thymocyte (T-T) interaction (T-T CD4+ T cells) is essential for predicting the characteristics of the antigens that bind to these T cells and for distinguishing T-T CD4+ T cells from other types of innate T cells. Using the TCRmini Tg mouse model, we show that the repertoire of TCRalpha chains in T-T CD4+ T cells was extremely diverse, in contrast to the repertoires previously described for other types of innate T cells. The TCRalpha chain sequences significantly overlapped between T-T CD4+ T cells and conventional CD4+ T cells in the thymus and spleen. However, the diversity of the TCRalpha repertoire of T-T CD4+ T cells seemed to be restricted compared with that of conventional CD4+ T cells. Interestingly, the frequency of the parental OT-II TCRalpha chains was significantly reduced in the process of T-T interaction. This diverse and shifted repertoire in T-T CD4+ T cells has biological relevance in terms of defense against diverse pathogens and a possible regulatory role during peripheral T-T interaction.
MeSH Terms
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Amino Acid Sequence
Animals
Antigens, Surface/metabolism
CD4-Positive T-Lymphocytes/cytology/*immunology/*metabolism
Cell Communication
Cell Differentiation/genetics/immunology
Clonal Evolution
Histocompatibility Antigens Class II/*immunology
*Immunity, Innate
Immunophenotyping
Lymphocyte Count
Mice
Mice, Knockout
Mice, Transgenic
Peptide Fragments/chemistry
Phenotype
Receptors, Antigen, T-Cell/chemistry/*genetics/metabolism
Receptors, Antigen, T-Cell, alpha-beta/chemistry/genetics
Spleen/cytology
Thymocytes/cytology/immunology/metabolism
Antigens, Surface
Histocompatibility Antigens Class II
Peptide Fragments
Receptors, Antigen, T-Cell
Receptors, Antigen, T-Cell, alpha-beta
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