Immune Netw.  2012 Oct;12(5):189-195. 10.4110/in.2012.12.5.189.

Vitamin C Induces Apoptosis in Human Colon Cancer Cell Line, HCT-8 Via the Modulation of Calcium Influx in Endoplasmic Reticulum and the Dissociation of Bad from 14-3-3beta

Affiliations
  • 1Department of Anatomy, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
  • 2Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Korea. genius29@snu.ac.kr

Abstract

It has been reported that vitamin C plays an effective role in the treatment and prevention of cancer, but its specific mechanisms are still largely unknown. The incidence of colon cancer is now increasing in Korea. Therefore, we have examined here the effect of vitamin C on the induction of the apoptosis on colon cancer and its related mechanisms. We have found that remarkable increase of the apoptosis and the calcium influx in endoplasmic reticulum (ER) in human colon cancer cell line, HCT-8. However, vitamin C-induced apoptosis was effectively inhibited by the pre-treatment of BAPTA-AM (1,2-bis(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid), which is well-known as a calcium specific chelator. During the apoptosis, we found the increase of the translocation of Bad to mitochondria from cytosol, after releasing from 14-3-3beta. In this process, the expression of Bax, a well-known pro-apoptotic protein, was also increased. Taken together, vitamin C induces apoptosis of colon cancer cell line, HCT-8 through the increase of 1) the calcium influx in endoplasmic reticulum (ER), 2) the translocation of Bad to mitochondria, and 3) the expression of Bax.

Keyword

Vitamin C; Colon cancer; Endoplasmic raticulum; Apoptosis; Bad; 14-3-3beta

MeSH Terms

Apoptosis
Ascorbic Acid
Calcium
Cell Line
Colon
Colonic Neoplasms
Cytosol
Dissociative Disorders
Egtazic Acid
Endoplasmic Reticulum
Glycine
Humans
Incidence
Korea
Mitochondria
Vitamins
Ascorbic Acid
Calcium
Egtazic Acid
Glycine
Vitamins
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