J Korean Ophthalmol Soc.  1997 May;38(5):805-816.

The Effect of Anticancerous Drug in the Fiboblast-Mediated Collagen Matrix Contraction

• Kwak HW
• Park CS
• Jin KH
• Kim JM

Abstract

In vitro PVR(Proliferative vitreoretinopathy) models allow identification of factor which may inhibit porliferation and contraction. In this study we evaluated the contraction of collagen matrix by choroidal fibroblast and the inhibition of contraction by antipoliferative drug. Each antiproliferative drug showed inhibition of collagen matrix contraction : Colchicine (0.1microgram/ml), Cytochalacin (0.05microgram/ml), Puromycin(10microgram/ml). Transmission electron microgram of collagen matrices containing colchicine, cytochalacin or puromycin showed no collagen fiber surrounding choroid fibroblast and showed cell destruction. Scanning electron microgram of collagen matrices containing colchicine, cytochalacin and puromycin showed that collagen fibers were well preserved without distortion. Colchicine, cytochalacin and puromycin are effective inhibitor of cell mediated contraction in additon to it`s potent antiproliferative effect wherease Interfereon has no anticontractile effect. The current study present a model to investigate the effect of antiproliferative drug on fibroblast mediated collagen matrix contraction.

Keyword

Proliferative vitreoretinopathy; colchicine; cytochalacin; puromycin; Interferon; collagen; fibroblast

MeSH Terms

Choroid
Colchicine
Collagen*
Fibroblasts
Interferons
Puromycin
Vitreoretinopathy, Proliferative
Colchicine
Collagen
Interferons
Puromycin