J Korean Ophthalmol Soc.  2007 Nov;48(11):1548-1553.

Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels

• Kim JW
• Chang WS
• Lee SY

Abstract

PURPOSE: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs).
METHODS
After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively.
RESULTS
Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05).
CONCLUSIONS
NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.

Keyword

Collagen gel; Contraction; Nitric oxide; Trabecular meshwork

MeSH Terms

Collagen*
Gels*
Humans
Nitric Oxide*
Nitroprusside
S-Nitroso-N-Acetylpenicillamine
Tissue Donors
Trabecular Meshwork*
Collagen
Gels
Nitric Oxide
Nitroprusside
S-Nitroso-N-Acetylpenicillamine

Figure

• 

  Figure 1. Effect of NO dcncrs (μM) cn the survival of trabecular meshwcrk cells embedded in the collagen gel measured with MTT assay. L-NAME and NO dcncrs did not affect cn the survival. (p>0.05)

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  Figure 2. Effect cf NO cbncrs (μM) cn the prcducticn cf NO measured with Griess assay. SNP and SNAP increased NO prcducticn significantly. (*; p<0.05)

• 

  Figure 3. Effect cf scdium nitrcprusside (SNP) cn the ccntracticn cf ccllagen gels. SNP inhibited the ccntracticn cf ccllagen gels significantly. (*; p<0.05, 10 μM) (jkos-48-1548; p<0.05, 100μM)

• 

  Figure 4. Effect cf SNAP cn the ccntracticn cf cc lagen gels. SNAP inhibited the ccntracticn cf ccllagen gels significantly in a time- and dcse-dependent manner. (*; p<0.05, 1μM) (jkos-48-1548; p<0.05, 10μM)

Reference

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