Nutr Res Pract.  2008 Dec;2(4):322-325. 10.4162/nrp.2008.2.4.322.

Kaempferol induced the apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 cells

• Choi EJ
• Ahn WS

• KMID: 2139210
• DOI: http://doi.org/10.4162/nrp.2008.2.4.322

Abstract

The aim of present study was to investigate the effects of kaempferol on cellular proliferation and cell cycle arrest and explore the mechanism for these effects in human breast carcinoma MDA-MB-453 cells. Cells were treated with kaempferol at various concentrations (ranging from 1 to 200 microM) for 24 and 48 hrs. Kaempferol significantly inhibited cancer cell growth in cells exposed to 50 and 10 microM of kaempferol and incubated for 24 and 48 hrs, respectively. Exposure to kaempferol resulted in cell cycle arrest at the G2/M phase. Of the G2/M-phase related proteins, kaempferol down-regulated CDK1 and cyclin A and B in cells exposed to kaempferol. In addition, small DNA fragments at the sub-G0 phase were increased by up to 23.12 and 31.90% at 10 and 50 microM incubated for 24 and 48 hrs, respectively. The kaempferol-induced apoptosis was associated with the up-regulation of p53. In addition, the phosphorylation of p53 at the Ser-15 residue was observed with kaempferol. Kaempferol inhibits cell proliferation by disrupting the cell cycle, which is strongly associated with the induction of arrest at G2/M phase and may induce apoptosis via p53 phosphorylation in human breast carcinoma MDA-MB-453 cells.

Keyword

Apoptosis; cell cycle arrest; kaempferol; p53; human breast carcinoma MDA-MB-453 cells

MeSH Terms

Apoptosis
Breast
Breast Neoplasms
Cell Cycle
Cell Cycle Checkpoints
Cell Proliferation
Cyclin A
DNA
Humans
Kaempferols
Phosphorylation
Proteins
Up-Regulation
Cyclin A
DNA
Kaempferols
Proteins

Figure

• 

  Fig. 1 Effect of kaempferol on cell proliferation of MDA-MB-453 cells. Cells were exposed to either vehicle (0.1% DMSO in medium) or kaempferol (1-200 µM) and incubated for 24 and 48 hrs. All data are reported as the percentage change in comparison with the vehicle-only group, which were arbitrarily assigned 100% viability. *P<0.05, significantly different from the vehicle-only group (kaempferol concentration="0").

• 

  Fig. 2 Effect of kaempferol on cell cycle distribution of MDA-MB-453 cells. Cells were exposed to either vehicle (0.1% DMSO in medium) or kaempferol at 50 and 10 µM for 24 and 48 hrs, respectively. Values are expressed as percentage of the cell population in the G1, S, and G1/M phase of cell cycle. *P<0.05, significantly different from the vehicle-only group (kaempferol concentration="0").

• 

  Fig. 3 Effect of kaempferol on protein expression related cell cycle arrest (A) and apoptosis (B) of human carcinoma MDA-MB-453 cells. Cells were exposed to either vehicle (0.1% DMSO in medium) or kaempferol at 50 µM for 24 hrs.

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