Korean J Obstet Gynecol.  2005 Mar;48(3):597-606.

Analysis of chromosomal DNA aberrations in Korean cervical squamous cell carcinoma cell lines by CGH

Affiliations
  • 1Department of Obstetrics and Gynecology Eulji Medical College, Daejon, Korea. ohky5@eulji.ac.kr
  • 2Department of Anatomy, Ewha Medical College, Seoul, Korea.
  • 3Department of Obstetrics and Gynecology, Ewha Medical College, Seoul, Korea.

Abstract


OBJECTIVE
Comparative genomic hybridization (CGH) is a new fluorescence in situ hybridization (FISH) technique to identify genomic aberrations in cancers. The purpose of this study was to analyze non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women.
METHODS
We analyzed non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women, SNU-17, SNU-682, and SNU-902 using CGH.
RESULTS
Chromosomal DNA gains of 5p, 5q22-q23, 8q11.2-q12, 14q21-qter, and 20 as well as chromosomal DNA losses of 21 were found frequently. Chromosomal DNA gains on chromosome 3q, 6P, 7p13-pter, 9p22-pter, 9q21-qter, 15q21-q22, 17q22-qter, 18p11.3-pter, 18q11.2-q21, 19p13.3-pter, 19q13.2-q13.3, and 22q12-qter, with losses on 4p14-pter, 10p11.2-p13 and 10q24 were observed in 2 of 3 cell lines.
CONCLUSION
Non-random aberrations which were disclosed in this study might be candidate regions for the abnormal genes involved in the tumorigenesis of cervical squamous cell carcinomas. Datas about chromosomal aberrations of Korean squamous cell carcinoma cell lines in this study could afford very useful basic information for the development of diagnostic and therapeutic modalties targeting the abnormal genes associated with uterine cervical cancer in Korea.

Keyword

Comparative genomic hybridization (CGH); Chromosomal DNA aberrations; Cervical squamous cell carcinoma cell lines

MeSH Terms

Carcinogenesis
Carcinoma, Squamous Cell*
Cell Line*
Chromosome Aberrations
Comparative Genomic Hybridization
DNA*
Female
Fluorescence
Humans
In Situ Hybridization
Korea
Uterine Cervical Neoplasms
DNA
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