Korean J Anat.  2005 Apr;38(2):145-151.

Analysis of Chromosomal Aberrations in Lung Cancer Cell Line, NCI-H1373

Affiliations
  • 1Department of Anatomy and Medical Research Institute, College of Medicine Ewha Womans University, Korea. psy03@ewha.ac.kr

Abstract

The cytogenetic analysis of recurring chromosomal aberrations play an important part to decide pathogenesis and prognosis of cancers. However, due to difficulties culturing tumor cells and complexity associated with the lesions, routine cytogenetic studies to analyze chromosomal imbalances are not sufficient. Comparative genomic hybridization (CGH) is a fluorescence in situ hybridization (FISH) technique to identify genomic imbalances in cancers, and array-CGH provides a method to measure the DNA copy-number changes quantitatively at an extremely high resolution and to map them directly onto the complete linear genome sequences. The purpose of this study was to confirm the utility of the CGH and array-CGH in analyzing chromosomal aberrations in lung cancer cell line, NCI-H1373, which was previously analyzed by karyotype analysis. The results of CGH and array-CGH in NCI-H1373 were similar to karyotype analysis. The array-CGH allowed us to pinpoint regions that were gained and lost. In this study, it was confirmed that CGH and array-CGH are an useful screening technique to analyze chromosomal aberrations in tumors.

Keyword

Genetic hybridization; Lung cancer; Chromosomal aberration

MeSH Terms

Cell Line*
Chromosome Aberrations*
Comparative Genomic Hybridization
Cytogenetic Analysis
Cytogenetics
DNA
Fluorescence
Genome
Hybridization, Genetic
In Situ Hybridization
Karyotype
Lung Neoplasms*
Lung*
Mass Screening
Prognosis
DNA
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