J Korean Surg Soc.  2010 Feb;78(2):111-115. 10.4174/jkss.2010.78.2.111.

Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients

• Moon SB
• Moon SH
• Park KJ

• KMID: 2096564
• DOI: http://doi.org/10.4174/jkss.2010.78.2.111

Abstract

PURPOSE
Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray.
METHODS
DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (M:F=15:2, mean age 35 years, 22~59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.
RESULTS
Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV.
CONCLUSION
Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.

Keyword

Anal condyloma acuminatum; Human papillomavirus; Human immunodeficiency virus; Polymerase chain reaction; DNA microarray

MeSH Terms

Cervix Uteri
Chimera
Coinfection
DNA
Female
HIV
Humans
Male
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
DNA

Figure

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  Fig. 1 Electrophoresis of the 1st PCR products (450 bp) and the nested PCR products (150 bp) (p = positive control, n = negative control).

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  Fig. 2 DNA chip scanning of patients No. 2, 5, 8.

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  Fig. 3 HPV type distribution in anal condyloma according to the HIV status (* = other type).

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  Fig. 4 Multiplicity of the HPV infection according to the HIV status.

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