Korean J Anesthesiol.  2010 Feb;58(2):153-161. 10.4097/kjae.2010.58.2.153.

Effect of propofol on cardiac function and gene expression after ischemic-reperfusion in isolated rat heart

• Kim YJ
• Lim HJ
• Choi SU

• KMID: 2092271
• DOI: http://doi.org/10.4097/kjae.2010.58.2.153

Abstract

BACKGROUND
The aim of this study was to examine the cardiac function and transcriptional response of the heart to propofol after ischemia-reperfusion. METHODS: Rat hearts were Langendorff-perfused using the modified Krebs-Henseleit buffer, and took 20 min stabilizing periods, 40 min ischemia periods, and then 120 min reperfusion period. The hearts were divided into 5 groups; Control: 180 min perfusion after stabilization, Ischemic: 40 min global ischemia after stabilization, followed by 120 min reperfusion, Pre: 2 micrometer propofol treatment was preformed only before ischemia, Post: 2 micrometer propofol treatment was performed only during reperfusion after ischemia, Pre/Post: 2 micrometer propofol treatment was performed both before and after ischemia. The measurement for cardiac performances, such as left ventricular developed pressure (LVDP), rate of left ventricular pressure generation (dP/dt), heart rate, and coronary flow were obtained. The expression profiles of isolated mRNA were determined by using Agilent microarray and real time-polymerase chain reaction (RT-PCR) was used to confirm the microarray results for a subset of genes. RESULTS: The Post group showed better LVDP and dP/dt than the Ischemic group. But there were no significant differences in heart rate and coronary flow among the groups. On the results of RT-PCR, the expressions of Abcc9, Bard1, and Casp4 were increased, but the expressions of Lyz, Casp8, and Timp1 were decreased in the Post group compared with the Ischemic group. CONCLUSIONS: This study suggests that 2 micrometer propofol may provide cardioprotective effect, and modulate gene expression such as apoptosis, and K(ATP) ion channel related-genes during reperfusion in the isolated rat hearts.

Keyword

Ischemia; Microarray; Propofol; Rat heart; Real time-polymerase chain reaction; Reperfusion

MeSH Terms

Animals
Apoptosis
Gene Expression
Glucose
Heart
Heart Rate
Ion Channels
Ischemia
Perfusion
Propofol
Rats
Reperfusion
RNA, Messenger
Tromethamine
Ventricular Pressure
Glucose
Ion Channels
Propofol
RNA, Messenger
Tromethamine