Korean J Clin Pathol.
1998 Jun;18(2):107-114.
Immunohistochemical Study on the Expression of Topoisomerase II alpha and Glutathione S-Transferase pi in Acute Myeloid Leukemia
- Affiliations
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- 1Department of Clinical Pathology, Pusan Hae Dong Hospital.
- 2Department of Clinical Pathology, College of Medicine, Pusan National University, Pusan, Korea.
- 3Department of Pathology, College of Medicine, Pusan National University, Pusan, Korea.
Abstract
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BACKGROUND: Topoisomerase II (topo II) is a major target of anthracyclines and epipodophyllotoxins for anticancer treatment. The expression of topo II is low in drug resistant cell lines. High levels of glutathione S-transferase (GST)pi have been associated with emergence of cell lines resistant to alkylating agents or adriamycin.
METHODS
By immunostaining with paraffin embedded bone marrow tissues, the expression of topo II alpha and GSTpi was investigated in 51 patients with acute myeloid leukemia (AML), and the relation of topo II alpha and GSTpi expression to treatment response in 29 patients with AML following induction chemotherapy was also evaluated.
RESULTS
Topo II positive cells varied from less than 1% to 60% of leukemic cells and 20 (39.2%) were negative for topo II (positive cells<10%). Treatment response following chemotherapy was not related to topo II. 26 (51.0%) were positive for GSTpi. GSTpi expression was related to treatment resistance of the patients following chemotherapy. In the patients who showed both topo II alpha negative and GSTpi positive, the frequency of treatment resistance following chemotherapy was high.
CONCLUSIONS
This study suggests that immunostaining of topo II alpha and GSTpi with the bone marrow paraffin sections of AML patients can be useful to predict the treatment response following chemotherapy and that further study including more patients with prospective study may substantiate topo II alpha and GSTpi as multidrug resistant markers.
Keyword
MeSH Terms
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Alkylating Agents
Anthracyclines
Bone Marrow
Cell Line
DNA Topoisomerases, Type II*
Doxorubicin
Drug Resistance, Multiple
Drug Therapy
Glutathione S-Transferase pi*
Glutathione Transferase*
Glutathione*
Humans
Immunohistochemistry
Induction Chemotherapy
Leukemia, Myeloid, Acute*
Paraffin
Podophyllotoxin
Alkylating Agents
Anthracyclines
DNA Topoisomerases, Type II
Doxorubicin
Glutathione
Glutathione S-Transferase pi
Glutathione Transferase
Paraffin
Podophyllotoxin
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