Korean J Dermatol.  2011 Mar;49(3):234-241.

Expression of Cyclin D1 and Bcl-xL in Basal Cell Carcinoma and Squamous Cell Carcinoma

• Kwon JI
• Kim SP
• Lee KS
• Cho JW

Abstract

BACKGROUND
Cyclin D1 has a key function in cell proliferation. Bcl-xL has anti-apoptotic effects that can protect against cell death. However, it is still largely unknown whether these proteins play important roles in development of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC).
OBJECTIVE
The purpose of this study was to investigate the expression of cyclin D1 and Bcl-xL in both of these carcinomas.
METHODS
In this study, we examined expression levels of cyclin D1 and Bcl-xL in both carcinomas by immunohistochemistry, immunofluorescence and Western blot analysis.
RESULTS
We found increased expression of both proteins in BCC and SCC using immunohistochemistry. Moreover, expression of cyclin D1 and Bcl-xL were increased in both carcinomas relative to normal skin or seborrheic keratosis in Western blot analysis. BCC showed greater increases in expression of cyclin D1 and Bcl-xL on peripheral lesions compared to the center of tumor nests.
CONCLUSION
That expression of cyclin D1 and Bcl-xL were increased in BCC and SCC, suggests that progression of the cell cycle combined with inhibition of apoptosis contributes to the carcinogenesis of BCC and SCC.

Keyword

Basal cell carcinoma; Bcl-xL; Cyclin D1; Squamous cell carcinoma

MeSH Terms

Apoptosis
Blotting, Western
Carcinoma, Basal Cell
Carcinoma, Squamous Cell
Cell Cycle
Cell Death
Cell Proliferation
Cyclin D1
Cyclins
Fluorescent Antibody Technique
Immunohistochemistry
Keratosis, Seborrheic
Proteins
Skin
Cyclin D1
Cyclins
Proteins