Korean J Obstet Gynecol.  2003 Oct;46(10):1989-1998.

The Study of the Cytotoxic Mechanism of Cisplatin in HeLa Cells

• Choi JH
• Cho H
• Lee SY
• Oh SH
• Kim HG
• Park RG

Abstract

Cis-diamminedichloroplatinum II (cisplatin) has been reported to induce cell death. However, the mechanism by which cisplatin is induced the apoptosis of cancer cells is still unclear. To evaluate the mechanistic insights of apoptosis by cisplatin, we tested the activities of apoptotic signaling pathway in HeLa cells. Apoptotic death of HeLa cells by cisplatin was confirmed by ladder-pattern fragmentation of genomic DNA. Cisplatin induced the activation of caspase-3 and 9 proteases in a time dependent manner. The caspase-3 protease activation and the cleavage of poly (ADP-ribose) polymerase (PARP) and procaspase-3 was demonstrated. We also showed that the expression of Bcl-2 and Bcl-xL was markedly decreased by the addition of cisplatin in HeLa cells. Moreover, expression of Fas and FasL proteins was increased by cisplatin. These data suggest that cisplatin triggers the activation of apoptotic signaling pathway in human cervical cancer, HeLa cells, via affects the expression of Fas, FasL and Bcl-2 families as well as triggers the activation of caspase-3 and -9 proteases.

Keyword

Cisplatin; HeLa cells; Apoptosis; Caspase-3 and 9 proteases

MeSH Terms

Apoptosis
Caspase 3
Cell Death
Cisplatin*
DNA
Fas Ligand Protein
HeLa Cells*
Humans
Peptide Hydrolases
Uterine Cervical Neoplasms
Caspase 3
Cisplatin
DNA
Fas Ligand Protein
Peptide Hydrolases