Korean J Obstet Gynecol.
2003 Dec;46(12):2410-2416.
Involvement of Oxidative Stress in Cisplatin-Induced Apoptosis in HeLa Cells
- Affiliations
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- 1Department of Obstetrics and Gynecology, College of Medicine, Wonkwang University, Iksan, Korea.
- 2Department of Microbiology, College of Medicine, Wonkwang University, Iksan, Korea.
Abstract
OBJECTIVE
To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined.
METHODS
Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry.
RESULTS
The pre-treatment with glutathione (GSH) significantly attenuated cisplatin-induced apoptosis through the reduction of reactive oxygen species (ROS) accumulation and diminished caspases-3 and 9 protease activity. Furthermore, z-VAD-fmk, an inhibitor of pan-caspase, effectively inhibited the activation of caspases and prevented apoptosis by cisplatin, although cisplatin-induced ROS generation was not attenuated.
CONCLUSION
These data indicate that ROS may play a role as an upstream mediator of caspases. Taken together, our results suggest that oxidative stress mediates cisplatin-induced apoptosis in HeLa cells.
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