J Korean Assoc Oral Maxillofac Surg.  2006 Apr;32(2):94-100.

Activation of NF-kappa B in the cisplatin-induced apoptosis of oral squamous cell carcinoma

• Seo JC
• Sung IY
• Kim JR

Abstract

Purpose: This study was done to confirm the role of NF-kappa B in cisplatin-induced apoptosis of oral squamous cell carcinoma.
Materials and Methods
Five cell lines originated from different oral cancer patients were tested for the apoptosis by the treatment of cisplatin. These cells showed different degree of cisplatin-resistance and the order is OSCC-2> OSCC-3> OSCC-5> OSCC-1> OSCC-4. OSCC-2 and OSCC-4 cells were assayed for the apoptosis by measuring DNA fragmentation and TUNEL staining after cisplatin treatment. While OSCC-4 cells showed apoptosis, OSCC-2 cells showed no or very slight apoptosis by cisplatin treatment. Next, It was determined whether NF-kappa B activation is required in mediating cisplatin-induced apoptosis of OSCC-4 . Result: The result was that elevated NF-kappa B activity mediated cisplatin-induced apoptosis.
Conclusion
In conclusion, these findings suggest that NF-kappa B activation is essential to cisplatin-induced apoptosis and it may be involved in cisplatin resistance in OSCC cells.

Keyword

Apoptosis; NF-kappa B; Cisplatin

MeSH Terms

Apoptosis*
Carcinoma, Squamous Cell*
Cell Line
Cisplatin
DNA Fragmentation
Humans
In Situ Nick-End Labeling
Mouth Neoplasms
Negotiating
NF-kappa B*
Cisplatin
NF-kappa B