Korean J Obstet Gynecol.  2003 Mar;46(3):647-650.

Effects of Magnesium sulfate on the expression of caspase-3 and caspase-9 of cytotrophoblasts under hypoxic condition

• Lee GS
• Kim SJ
• Hur M
• Park SH
• Lee HJ
• Lee WS
• Shin JC

Abstract

OBJECTIVE
To determine the effect of magnesium sulfate (MgSO4) on the expression of caspase-3 and caspase-9 of cytotrophoblasts in vitro under normal and hypoxic condition as assessed immunoblot analyses.
METHODS
Normal cytotrophoblasts were isolated from second trimester placentas and cultured in several physiologically relevant concentrations of MgSO4 under standard tissue culture condition (20% O2) or hypoxic condition (1-2% O2). Cytotrophoblasts apoptosis was estimated by TUNEL staining and by immunoblotting for Caspase-9 and Caspase-3.
RESULTS
The apoptotic index was highest in cytotrophoblasts cultured under hypoxic conditions for 36 hour in the absence of MgSO4 and was showed decreasing tendency by the addition of MgSO4 under the same condition. The expression of Caspase-9 did not change under both standard condition and hypoxic condition with increasing MgSO4 concentrations, but the expression of Caspase-3 decreased under hypoxic condition with increasing of MgSO4 concentrations.
CONCLUSION
MgSO4 might protect cytotrophoblasts from apoptosis under hypoxic condition.

Keyword

MgSO4; Cytotrophoblast; Caspase-3; Caspase-9; Hypoxia

MeSH Terms

Anoxia
Apoptosis
Caspase 3*
Caspase 9*
Female
Humans
Immunoblotting
In Situ Nick-End Labeling
Magnesium Sulfate*
Magnesium*
Placenta
Pregnancy
Pregnancy Trimester, Second
Trophoblasts*
Caspase 3
Caspase 9
Magnesium
Magnesium Sulfate