Korean J Perinatol.
2004 Sep;15(3):262-267.
Effects of magnesium sulfate on VEGF and Caspase-3 of HUVECs (Human umbilical vein endothelial cells)
- Affiliations
-
- 1Department of Obstetrics and Gynecology, College of Medicine, Catholic University of Korea, Seoul, Korea. leegsr@catholic.ac.kr
- 2Department of Pediatrics, College of Medicine, Catholic University of Korea, Seoul, Korea.
- 3Research Center for Clinical Medicine, Holy Family Hospital, Catholic University, Pucheon, Korea.
Abstract
OBJECTIVES
To determine the effects of magnesium sulfate on Caspase-3 and Vascular endothelial growth factor (VEGF) of human umbilical vein endothelial cells (HUVECs) under presence or absence of Tumor necrosis factor (TNF-alpha).
METHODS
HUVECs were isolated from normal term umbilical cords and cultured in several physiolo gically relevant concentrations of magnesium sulfate with or without exposure of TNF-alpha. The concentrations of VEGF and caspase-3 were estimated by colorimetric assay and ELISA assay, respectively.
RESULTS
The concentration of VEGF in HUVECs significantly increased in the presence of TNF-alpha compared with in the absence of TNF-alpha. However, the concentration of VEGF did not show significant difference in several concentrations of magnesium sulfate concentrations with addition of TNF-alpha and it showed the lowest concentration under 4 mM and 8 mM of magnesium sulfate concentration without addition of TNF-alpha. The concentration of caspase-3 in HUVECs did not show statistically significant difference with the addition of TNF-alpha and magnesium sulfate.
CONCLUSION
TNF-alpha induce HUVECs to stimulate the VEGF, and magnesium sulfate might not have the effects on the expression of VEGF with addition of TNF-alpha However, the concentration for treatment of magnesium sulfate inhibits the expression of VEGF without addition of TNF-alpha. Magnesium sulfate might not have an effect of the expression of caspase-3 with or without addition of TNF-alpha.
Keyword
MeSH Terms
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Caspase 3*
Enzyme-Linked Immunosorbent Assay
Human Umbilical Vein Endothelial Cells
Magnesium Sulfate*
Magnesium*
Tumor Necrosis Factor-alpha
Umbilical Cord
Umbilical Veins*
Vascular Endothelial Growth Factor A*
Caspase 3
Magnesium
Magnesium Sulfate
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A
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