Korean J Obstet Gynecol.  2003 Oct;46(10):2028-2032.

Effects of Magnesium Sulfate on the Apoptosis and Invasion of Cytotrophoblasts under Hypoxic Condition

• Lee GS
• Kim SJ
• Hur SY
• Kwon I
• Park IY
• Park SH
• Shin JC
• Kim SP

Abstract

OBJECTIVE
To determine the effect of magnesium sulfate (MgSO4) on the apoptosis and invasion of cytotrophoblasts in vitro under normal and hypoxic condition as assessed immunoblot analyses of Bcl-2/Bax, invasion assay and immunohistochemical staining of integrin alpha1.
METHODS
Normal cytotrophoblasts were isolated from second trimester placentas and cultured in several physiologically relevant concentrations of MgSO4 under control tissue culture condition (20% O2) or hypoxic condition (1-2% O2). Apoptosis of cytotrophoblasts was estimated by immunoblotting for Bcl-2/Bax, invasiveness was estimated by invasion assay and immunohistochemical staining of Integrin alpha1.
RESULTS
The expression of Bcl-2 did not change under standard condition, but it decreased under hypoxic condition with increasing of MgSO4 concentrations. The expression of Bax did not change under both standard condition and hypoxic condition with increasing MgSO4 concentrations. The invasiveness of cytotrophoblasts significantly decreased under both control and hypoxic conditions with increasing of MgSO4 concentrations. The expression of Integrin alpha1 immumohistochemical staining significantly decreased under control condition and showed decreasing tendency under hypoxic condition with increasing of MgSO4 concentrations.
CONCLUSION
MgSO4 might induce cytotrophoblasts to the apoptosis and inhibit invasion of cytotrophoblasts under hypoxic condition.

Keyword

MgSO4; Cytotrophoblast; Bcl-2/Bax; Invasion; Integrin alpha1

MeSH Terms

Apoptosis*
Female
Humans
Immunoblotting
Integrin alpha1
Magnesium Sulfate*
Magnesium*
Placenta
Pregnancy
Pregnancy Trimester, Second
Trophoblasts*
Integrin alpha1
Magnesium
Magnesium Sulfate