Korean J Otolaryngol-Head Neck Surg.  2002 Oct;45(10):984-989.

The Change of Molecular Event of p53 by Cisplatin and 5-Fluorouracil in Hypopharyngeal Cell Line(PNUH-12)

• Chon KM
• Lee BJ
• Lee IW
• Moon YI
• Roh HJ
• Wang SG
• Goh EK
• Kim SR
• Lee EY

Abstract

OBJECTIVES
AND BACKGROUND: In head and neck cancer including hypopharyngeal carcinoma, cisplatin and 5-fluorouracil usually have been used as neoadjuvant chemotherapeutic agents. We investigated the difference in the influences of cisplatin and 5-fluorouracil (5-FU) on the p53 protein expression and cell responses (cell cycle arrest and apoptosis) in the hypopharyngeal cell line (PHUH-12). METHOD: PNUH-12 with a mutant type p53 (one point mutation at the 78th base, C to G, in exon 7) was treated with cisplatin and 5-FU. Changes in the cell line were assessed by MTT assay, Western blotting (p53 and p21 protein), DNA fragmentation, PI stain, and DNA flow cytometry.
RESULTS
The p53 protein expression was increased after the treatment with cisplatin and 5-FU. The expression of p21 protein was increased after the treatment with 5-FU, not cisplatin. With cisplatin, we observed apoptosis by DNA fragmentation and PI stain and the increased S phase on DNA flow cytometry. But, with 5-FU, we couldn't observe apoptosis by DNA fragmentation, PI, and flow cytometry and only the increased G1 phase on DNA flow cytometry.
CONCLUSION
In hypopharyngeal cell line (PNUH-12), cisplatin induced p53 dependent apoptosis and 5-FU induced p53 and p21 dependent G0/G1 cell cycle arrest, but not apoptosis.

Keyword

Cisplatin; Fluorouracil; Hypopharyngeal neoplasms

MeSH Terms

Apoptosis
Blotting, Western
Cell Cycle Checkpoints
Cell Line
Cisplatin*
DNA
DNA Fragmentation
Exons
Flow Cytometry
Fluorouracil*
G1 Phase
Head and Neck Neoplasms
Hypopharyngeal Neoplasms
Point Mutation
S Phase
Cisplatin
DNA
Fluorouracil