Korean J Otolaryngol-Head Neck Surg.  2004 May;47(5):448-451.

The Change of Telomerase Activity by Cisplatin and 5-FU in Head and Neck Cancer Cell Lines

Affiliations
  • 1Department of Otolaryngology, College of Medicine, Pusan National University, Pusan, Korea.
  • 2Department of Biochemistry, College of Medicine, Pusan National University, Pusan, Korea.

Abstract

BACKGROUND AND OBJECTIVES
Telomerase activity appears to be associated with cell immortalisation and malignant progres-sion. Telomerase activity has been found in the majority of human tumours, but not in most somatic cells or tissues. We studied the change in the telomerase activity by cisplatin and 5-fluorouracil (5-FU) in head and neck cancer cell lines. SUBJECTS AND METHODS: Three different human head and neck cancer cell lines (PNUH-12, SNU-899, HEp-2) were studied. Cell cytotoxi-city and change of telomerase activity by 5-FU and cisplatin treatment were assessed by MTT and TRAP assays. RESULTS: The telomerase activity was detected in the head and neck cancer cell lines. After the cisplatin treatment, the telomerase activity was decreased in a concentration-dependent manner. However, after 5-FU treatment, the telomerase activity showed a slight decrease or no change at the concentration similar to that of cisplatin in the inhibition of tumor growth. CONCLUSION: Our results can explain one of the reasons why cisplatin is more effective than 5-FU in the chemotherapy of head and neck cancer.

Keyword

Telomerase; Cisplatin; Fluorouracil; Head and neck neoplasms

MeSH Terms

Cell Line*
Cisplatin*
Drug Therapy
Fluorouracil*
Head and Neck Neoplasms*
Head*
Humans
Telomerase*
Cisplatin
Fluorouracil
Telomerase
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