Korean J Otorhinolaryngol-Head Neck Surg.
2008 May;51(5):422-428.
Neural Differentiation of Mesenchymal Stem Cells from Bone Marrow of Human Mastoid Process
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwangju, Korea. choyb@chonnam.ac.kr
- 2Department of Physiology, Chonnam National University Medical School, Gwangju, Korea.
- 3Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: Reports of neural differentiation of mesenchymal stem cells suggest the possibility that these cells may serve as a source for stem cell-based regenerative medicine to treat neurological disorders. The purpose of this study was to generate neural cells by differentiation of bone marrow-derived mesenchymal stem cells that isolated from human mastoid process.
MATERIALS AND METHOD: Human mesenchymal stem cells (hMSCs) isolated from human mastoid process bone marrow during mastoidectomy for chronic otitis media surgery were characterized using fluorescence-activated cell sorter. Induction of neural differentiation from hMSCs was performed using mitogenic factors (basic fibroblast growth factor, epidermal growth factor, forskolin, isobutylmethylxanthine), and the characterization of differentiated hMSCs was performed using immunohistochemistry, RT-PCR and whole cell patch clamp technique.
RESULTS
hMSCs from bone marrow of mastoid process were isolated and cultured. Differentiated cells from hMSCs expressed mRNA transcripts for neuron specific markers, TUJ1 and neurofilament proteins (NF-L, NF-M) as determined by RT-PCR, and neuron specific markers, suhc as NeuN, TUJ1, microtubule-associated protein-2 (MAP2) and glial fibrillary acidic protein by immunohistochemistry. These cells showed voltagedependent sodium currents that was blocked by tetrodotoxin.
CONCLUSION
hMSCs, which were isolated from human mastoid process bone marrow, were one of the good sources for stem cell-based regenerative medicine to treat neurological disorders.
MeSH Terms
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Bone Marrow
Cell Differentiation
Epidermal Growth Factor
Fibroblast Growth Factors
Forskolin
Glial Fibrillary Acidic Protein
Humans
Immunohistochemistry
Mastoid
Mesenchymal Stromal Cells
Nervous System Diseases
Neurofilament Proteins
Neurons
Otitis Media
Regenerative Medicine
RNA, Messenger
Sodium
Temporal Bone
Epidermal Growth Factor
Fibroblast Growth Factors
Forskolin
Glial Fibrillary Acidic Protein
Neurofilament Proteins
RNA, Messenger
Sodium
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