Korean J Pediatr Hematol Oncol.  2001 Oct;8(2):314-322.

Establishment and Management of Pediatric Tumor Tissue Bank

• Jung HL
• Kim DH
• Kim SY
• Yoo KH
• Sung KW
• Koo HH
• Lee SK
• Shin HJ
• Kim JG
• Yoon HK

Abstract

PURPOSE: Current pediatric cancer research requires an organized pediatric tumor tissue bank with standardized guidelines for preparation and storage of human tumor tissue samples, white cells, serum, genomic DNA, RNA, cDNA and proteins.. Our institution established and managed pediatric tumor tissue bank for the last one year, and we want to present an overview of our experiences and guidelines.
METHODS
From leukemia patients, peripheral blood and bone marrow aspirates were collected at initial diagnosis. Leukemic cells were prepared by Ficoll density-gradient centrifugation and stored at 196oC liquid nitrogen. For solid tumors, tissue cultures were performed as soon as possible after surgical excision or needle biopsy. Serum free media and primary cultured cells were collected and stored at 20degrees C and at 196degrees C, respectively. Genomic DNA, RNA and cDNA were isolated from leukemic cells and cultured solid tumor cells, and stored at 20degrees C. We also isolated genomic DNA from white blood cells of solid tumor patients and stored at 20degrees C. Finally we collected serum samples from all pediatric cancer patients at diagnosis and stored at 20degrees C.
RESULTS
Among the 41 cases of leukemia and 100 cases of solid tumor patients who were diagnosed at department of pediatrics, Samsung Medical Center, from August 2000 to July 2001, 26 cases (63%) of leukemia and 59 cases (59%) of solid tumor patients were registered to Pediatric Tumor Tissue Bank. Primary cell cultures were performed in 21 cases of solid tumors and were successful in 19 cases (90%). The isolated genomic DNA, RNA and cDNA were all in high quality confirmed by electrophoresis in agarose gel.
CONCLUSION
The problem of tissue sample size obtained by needle biopsy could be overcome by primary cell cultures. For the effective management of pediatric tumor tissue bank, fresh tissue collection with active cooperation of surgeons, organized personnel structure, and multidisciplinary standardized guidelines are necessary.

Keyword

Pediatric tumor tissue bank; Leukemia; Solid tumor; Cell culture; DNA; RNA; cDNA

MeSH Terms

Biopsy, Needle
Bone Marrow
Cell Culture Techniques
Cells, Cultured
Centrifugation
Culture Media, Serum-Free
Diagnosis
DNA
DNA, Complementary
Electrophoresis
Ficoll
Humans
Leukemia
Leukocytes
Nitrogen
Pediatrics
Primary Cell Culture
RNA
Sample Size
Sepharose
Tissue Banks*
Culture Media, Serum-Free
DNA
DNA, Complementary
Ficoll
Nitrogen
RNA
Sepharose