J Bacteriol Virol.  2010 Sep;40(3):103-109. 10.4167/jbv.2010.40.3.103.

Identification of Helicobacter pylori Strain 51 Major Outer Membrane Proteins by Quadrupole Time of Flight Mass Spectrometry

• Kim KM
• Lee SG
• Cho YA
• Song YG
• Song JY
• Kang HL
• Lee WK
• Cho MJ
• Rhee KH
• Baik SC

• KMID: 2055047
• DOI: http://doi.org/10.4167/jbv.2010.40.3.103

Abstract

As part of an initial inquiry into the function of the outer membrane proteins (OMPs) of Helicobacter pylori Korean strain 51, we have conducted an extensive proteome analysis via quadrupole time of flight (Q-TOF) mass spectrometry (MS). Fifty one OMPs of H. pylori were purified using sarcosine and resolved via two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were observed in the alkaline pI regions (6.0~11.0) at molecular masses between 10~100 KDa. Here, 15 spots were identified, representing 9 types of genes (KHP0852, KHP0853, KHP1353, KHP1017, KHP0172, KHP0076, KHP0617, KHP1069, KHP0614) from the sarcosin-insoluble fraction of H. pylori 51. These may be employed in the characterization of the OMPs of H. pylori 51, which will help to identify new potential target proteins for vaccine development and drug therapy.

Keyword

Helicobacter pylori; Outer membrane protein; Q-TOF MS

MeSH Terms

Electrophoresis
Helicobacter
Helicobacter pylori
Mass Spectrometry
Membrane Proteins
Membranes
Proteins
Proteome
Proton-Motive Force
Sarcosine
Sprains and Strains
Membrane Proteins
Proteins
Proteome
Sarcosine

Figure

• 

  Figure 1. H. pylori strain 51 outer membrane protein 2-DE map. The sarcosyl-insoluble proteins were separated on an IPG strip and detected via silver staining. The proteins of the circled spots were identified by Q-TOF.

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