J Bacteriol Virol.  2006 Jun;36(2):89-98. 10.4167/jbv.2006.36.2.89.

Host Gene Profiling of Coxsackievirus B3 H3- and 10A1-infected Mouse Heart

Affiliations
  • 1Department of Biotechnology, The Catholic University of Korea, Korea. jhnam@catholic.ac.kr
  • 2Department of Medicine, Sunkyunkwan University School of Medicine, Cardiac and Vascular Center, Samsung Medical Center, seoul, Korea.
  • 3Division of Hepatitis and Poliovirus, Department of Virology, National Institute of Health, Seoul, Korea.

Abstract

Coxsackievirus B3 (CVB3) is a non-enveloped virus that has a single-stranded RNA genome. CVB3 induces myocarditis, and ultimately, dilated cardiomyopathy. A myocarditis variant of CVB3 (CVB3 H3) and its antibody-escape mutant (CVB3 10A1) were studied previously; H3 was found to induce myocarditis and 10A1 was found to be attenuated in infected mice. Although amino acid residue 165, located in a puff region of VP2, was found to be altered (i.e., the H3 asparagine was altered to aspartate in 10A1), the detailed mechanism of attenuation was not clearly elucidated. Here, DNA microarray technology was used to monitor changes in mRNA levels of infected mouse hearts after CVB3 H3 and 10A1 infection. This tool was used to elucidate the pathogenic mechanisms of viral infection by understanding virus-host interactions. We identified several genes, including protein tyrosine kinases, Ddr2 and Ptk2, as well as Clqb and Crry, involved in complement reactions, which may be involved in these viral processes. Thus, gene profiling can provide an opportunity to understand host immune responses to viral infection for gene therapy and may contribute to the identification of the target gene that is modified during treatment of viral myocarditis.

Keyword

Coxsackievirus B3 H3; 10A1; cDNA microarray; Gene profile

MeSH Terms

Animals
Asparagine
Aspartic Acid
Cardiomyopathy, Dilated
Complement System Proteins
Genetic Therapy
Genome
Heart*
Mice*
Myocarditis
Oligonucleotide Array Sequence Analysis
Protein-Tyrosine Kinases
RNA
RNA, Messenger
Asparagine
Aspartic Acid
Complement System Proteins
Protein-Tyrosine Kinases
RNA
RNA, Messenger
Full Text Links
  • JBV
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error