Mycobiology.
2009 Sep;37(3):230-237. 10.4489/MYCO.2009.37.3.230.
Isolation of Genes Specifically Expressed in Different Developmental Stages of Pleurotus ostreatus Using Macroarray Analysis
- Affiliations
-
- 1Bio-crops Development Division, National Academy of Agricultural Science, RDA, Suwon, Korea. bgkimpeace@gmail.com
- 2Department of Applied Biochemistry, College of Natural Science, Konkuk University, Chung-Ju, Korea.
- 3Research Institute of Narae Bioteh, Naraebio Tech Inc., Gun-po City, Korea.
- 4Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, Korea.
- KMID: 2053019
- DOI: http://doi.org/10.4489/MYCO.2009.37.3.230
Abstract
- The oyster mushroom (Pleurotus ostreatus) is one of the most important edible mushrooms worldwide. The mechanism of P. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. Based on our previous publication of P. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental stages of P. ostreatus. Gene expression profile databases generated by evaluating expression levels showed that 33, 10, and 94 genes were abundantly expressed in mycelium, fruiting body and basidiospore developmental stages, respectively. Among them, the genes specifically expressed in the fruiting body stage were further analyzed by reverse transcription-polymerase chain reaction and Northern blot to investigate temporal and spatial expression patterns. These results provide useful information for future studies of edible mushroom development.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Differential Expression of Laccase Genes in Pleurotus ostreatus and Biochemical Characterization of Laccase Isozymes Produced in Pichia pastoris
- Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer
- The Efficient Transformation of Pleurotus ostreatus using REMI Method
- Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation
- Characterization of Fruitbody Morphology on Various Environmental Conditions in Pleurotus ostreatus
