Mycobiology.  2004 Jun;32(2):88-94. 10.4489/MYCO.2004.32.2.088.

Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation

• Joh JH
• Kim BG
• Kong WS
• Yoo YB
• Chu KS
• Kim NK
• Park HR
• Cho BG
• Lee CS

• KMID: 1516940
• DOI: http://doi.org/10.4489/MYCO.2004.32.2.088

Abstract

Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant phenotypes in mycelia and fruiting processes. The variant phenotypes were classified into 6 groups: (1) auxotrophic strains, which are incapable of growing on minimal media and can only grow when provided with their specific requirements; (2) abnormal vegetative strains, which grow very slowly on minimal and complete media; (3) primordiumless strains, which fail to develop to the formation of primordia; (4) maturationless strains, which form primordia, but do not form mature fruiting bodies; (5) specifically colored strains, which have Specific bluish grey or bluish white pileus; (6) poorly spored strains, which fail to produce basidiospore or which produce few spores. These variant strains may be useful in genetic breeding programs and for the studies of fungal development and genetics.

Keyword

Color of pileus; Developmental mutants; Pleurotus ostreatus; UV mutagenesis; auxotrophs

MeSH Terms

Breeding
Fruit
Genetics
Phenotype
Pleurotus*
Protoplasts
Spores
Ultraviolet Rays