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Molecular Marker Related to Fruitbody Color of Flammulina velutipes

Kong WS, You CH, Yoo YB, Kim GH, Kim KH

  • KMID: 2312424
  • Mycobiology.
  • 2004 Mar;32(1):6-10.
White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And...
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Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer

Park YJ, Yoon DE, Kim HI, Kwon OC, Yoo YB, Kong WS, Lee CS

  • KMID: 2312745
  • Mycobiology.
  • 2014 Jun;42(2):193-197.
Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of...
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Taxonomic Position and Species Identity of the Cultivated Yeongji 'Ganoderma lucidum' in Korea

Kwon OC, Park YJ, Kim HI, Kong WS, Cho JH, Lee CS

Ganoderma lucidum has a long history of use as a traditional medicine in Asian countries. However, the taxonomy of Ganoderma species remains controversial, since they were initially classified on the...
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Breeding of Flammulina velutipes Strains Adaptable to Elevated-temperature

Kong WS, Cho YH, Jhune CS, Yoo YB, Kim KH

  • KMID: 2312425
  • Mycobiology.
  • 2004 Mar;32(1):11-16.
Winter mushroom, Flammulina velutipes, needs low temperature during its cultivation. To save on farm costs, especially during summer, a strain adaptable to a higher or elevated-temperature must be developed. At...
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The Efficient Transformation of Pleurotus ostreatus using REMI Method

Joh JH, Kim BG, Chu KS, Kong WS, Yoo YB, Lee CS

Restriction enzyme-mediated integration (REMI) was used to transform uracil auxotrophs of Pleurotus ostreatus to prototrophy. When protoplasts of Pleurotus ostreatus were treated by the reaction mixture containing 10 units of...
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Evaluating Genetic Diversity of Agaricus bisporus Accessions through Phylogenetic Analysis Using Single-Nucleotide Polymorphism (SNP) Markers

Oh YL, Choi IG, Kong WS, Jang KY, Oh Mj, Im JH

Agaricus bisporus, commonly known as the button mushroom, is widely cultivated throughout the world. To breed new strains with more desirable traits and improved adaptability, diverse germplasm, including wild accessions,...
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Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation

Joh JH, Kim BG, Kong WS, Yoo YB, Chu KS, Kim NK, Park HR, Cho BG, Lee CS

Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant...
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Analysis of Cultural Characteristics and Phylogenic Relationships of Collected Strains of Pholiota species

Cho YH, Kong WS, Kim GH, Jhune CS, You CH, Yoo YB, Kim KH

  • KMID: 2312413
  • Mycobiology.
  • 2003 Dec;31(4):200-204.
Cultural characteristics and phylogenic relationships were investigated and classified among collected strains in Pholiota spp. which contain P. adiposa, P. squarrosa, P. nameko etc. They were tested on the four...
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Ribosomal Intergenic Spacer 1 Based Characterization of Button Mushroom (Agaricus bisporus) Strains

Kwon HW, Choi MA, Kim DW, Oh YL, Hyun MW, Kong WS, Kim SH

Breeding the button mushroom requires genetic information about its strains. This study was undertaken to genetically characterize four domestically bred button mushroom strains (Saea, Saejung, Saedo, Saeyeon cultivars) and to...
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Genome-Wide Comparison of Carbohydrate-Active Enzymes (CAZymes) Repertoire of Flammulina ononidis

Park YJ, Kong WS

Whole-genome sequencing of Flammulina ononidis, a wood-rotting basidiomycete, was performed to identify genes associated with carbohydrate-active enzymes (CAZymes). A total of 12,586 gene structures with an average length of 2009 bp...
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Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes

Kim HI, Kwon OC, Kong WS, Lee CS, Park YJ

The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome,...
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Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)

Kwon HW, Choi MA, Yun YH, Oh YL, Kong WS, Kim SH

To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038...
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Development of 3-D Radiosurgery Planning System Using IBM Personal Computer

Suh TS, Suh DY, Park CI, Ha SW, Kong WS, Park SH, Yoon SC

  • KMID: 2196919
  • J Korean Soc Ther Radiol.
  • 1993 Jun;11(1):167-174.
Recently, stereotactic radiosurgery plan is required with the information of 3-D image and dose distribution. A project has been doing if developing LINAC based stereotactic radiosurgery since April 1991. The...
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Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

An H, Jo IH, Oh YL, Jang KY, Kong WS, Sung JK, So YS, Chung JW

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic...
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Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes

Yun YH, Koo JS, Kim SH, Kong WS

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to...
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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Kim JK, Park YJ, Kong WS, Kang HW

  • KMID: 2312600
  • Mycobiology.
  • 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using...
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Development of a Molecular Marker Linked to the A4 Locus and the Structure of HD Genes in Pleurotus eryngii

Lee SH, Ali A, Ha B, Kim MK, Kong WS, Ryu JS

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier...
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Development of Polymorphic Simple Sequence Repeat Markers using High-Throughput Sequencing in Button Mushroom (Agaricus bisporus)

Lee HY, Raveendar , An H, Oh YL, Jang KY, Kong WS, Ryu H, So YS, Chung JW

The white button mushroom (Agaricus bisporus) is one of the most widely cultivated species of edible mushroom. Despite its economic importance, relatively little is known about the genetic diversity of...
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Screening of Biodegradable Function of Indigenous Ligno-degrading Mushroom Using Dyes

Jang KY, Cho SM, Seok SJ, Kong WS, Kim GH, Sung JM

The process of biodegradation in lingo-cellulosic materials is critically relevant to biospheric carbon. The study of this natural process has largely involved laboratory investigations, focused primarily on the biodegradation and...
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