Mycobiology.  2018 Dec;46(4):349-360. 10.1080/12298093.2018.1537585.

Genome-Wide Comparison of Carbohydrate-Active Enzymes (CAZymes) Repertoire of Flammulina ononidis

• Park YJ
• Kong WS

• KMID: 2434563
• DOI: http://doi.org/10.1080/12298093.2018.1537585

Abstract

Whole-genome sequencing of Flammulina ononidis, a wood-rotting basidiomycete, was performed to identify genes associated with carbohydrate-active enzymes (CAZymes). A total of 12,586 gene structures with an average length of 2009"‰bp were predicted by the AUGUSTUS tool from a total 35,524,258"‰bp length of de novo genome assembly (49.76% GC). Orthologous analysis with other fungal species revealed that 7051 groups contained at least one F. ononidis gene. In addition, 11,252 (89.5%) of 12,586 genes for F. ononidis proteins had orthologs among the Dikarya, and F. ononidis contained 8 species-specific genes, of which 5 genes were paralogous. CAZyme prediction revealed 524 CAZyme genes, including 228 for glycoside hydrolases, 21 for polysaccharide lyases, 87 for glycosyltransferases, 61 for carbohydrate esterases, 87 with auxiliary activities, and 40 for carbohydrate-binding modules in the F. ononidis genome. This genome information including CAZyme repertoire will be useful to understand lignocellulolytic machinery of this white rot fungus F. ononidis.

Keyword

Flammulina ononidis; genome; carbohydrate-active enzyme

MeSH Terms

Basidiomycota
Esterases
Flammulina*
Fungi
Genome
Glycoside Hydrolases
Glycosyltransferases
Polysaccharide-Lyases
Esterases
Glycoside Hydrolases
Glycosyltransferases
Polysaccharide-Lyases