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Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Cho JH, Lee SE, Chang WB, Cha JS

  • KMID: 2312487
  • Mycobiology.
  • 2006 Jun;34(2):104-107.
Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest...
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Molecular Marker Related to Fruitbody Color of Flammulina velutipes

Kong WS, You CH, Yoo YB, Kim GH, Kim KH

  • KMID: 2312424
  • Mycobiology.
  • 2004 Mar;32(1):6-10.
White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And...
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Breeding of Flammulina velutipes Strains Adaptable to Elevated-temperature

Kong WS, Cho YH, Jhune CS, Yoo YB, Kim KH

  • KMID: 2312425
  • Mycobiology.
  • 2004 Mar;32(1):11-16.
Winter mushroom, Flammulina velutipes, needs low temperature during its cultivation. To save on farm costs, especially during summer, a strain adaptable to a higher or elevated-temperature must be developed. At...
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Cloning and Characterization of a Novel Laccase Gene, fvlac7, Based on the Genomic Sequence of Flammulina velutipes

Kim JK, Lim SH, Kang HW

  • KMID: 2312683
  • Mycobiology.
  • 2013 Mar;41(1):37-41.
Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on...
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Genome-Wide Comparison of Carbohydrate-Active Enzymes (CAZymes) Repertoire of Flammulina ononidis

Park YJ, Kong WS

Whole-genome sequencing of Flammulina ononidis, a wood-rotting basidiomycete, was performed to identify genes associated with carbohydrate-active enzymes (CAZymes). A total of 12,586 gene structures with an average length of 2009 bp...
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Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes

Kim HI, Kwon OC, Kong WS, Lee CS, Park YJ

The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome,...
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Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes

Yun YH, Koo JS, Kim SH, Kong WS

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to...
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Determination of Mineral Components in the Cultivation Substrates of Edible Mushrooms and Their Uptake into Fruiting Bodies

Lee CY, Park JE, Kim BB, Kim SM, Ro HS

  • KMID: 2408780
  • Mycobiology.
  • 2009 Jun;37(2):109-113.
The mineral contents of the cultivation substrates, fruiting bodies of the mushrooms, and the postharvest cultivation substrates were determined in cultivated edible mushrooms Pleurotus eryngii, Flammulina velutipes, and Hypsizigus marmoreus....
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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Kim JK, Park YJ, Kong WS, Kang HW

  • KMID: 2312600
  • Mycobiology.
  • 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using...
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Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2)

Kim JS, Chung HY, Na K

  • KMID: 2313311
  • Nutr Res Pract.
  • 2007 Sep;1(3):180-183.
Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe...
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Identification and Characterization of Two New S-Adenosylmethionine-Dependent Methyltransferase Encoding Genes Suggested Their Involvement in Stipe Elongation of Flammulina velutipes

Huang Q, Mukhtar I, Zhang Y, Wei Z, Han X, Huang R, Yan J, Xie B

Two new SAM-dependent methyltransferase encoding genes (fvsmt1 and fvsmt2) were identified from the genome of Flammulina velutipes. In order to make a comprehensive characterization of both genes, we performed in...
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Characterization of Species of Cladobotryum which Cause Cobweb Disease in Edible Mushrooms Grown in Korea

Back CG, Lee CY, Seo GS, Jung HY

  • KMID: 2312672
  • Mycobiology.
  • 2012 Sep;40(3):189-194.
Four Cladobotryum isolates were collected from four different commercially grown mushroom types infected with cobweb disease in Cheongdo-gun and Chilgok-gun of Gyeongbuk Province, Korea in 2010. The isolates were identified...
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A Survey on Preferences for Vegetable Cooking Methods and Vegetable-aversion-related Factors among Elementary School Students in Kwangju and Chonnam Regions

Ahn YK, Ro HK

  • KMID: 2300933
  • Korean J Community Nutr.
  • 2009 Oct;14(5):531-544.
This study was conducted to survey multiple factors of aversion to vegetables and preferences for vegetable-related recipes in school meal services among elementary school children in order to help develop...
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