Mycobiology.  2019 Dec;47(4):527-532. 10.1080/12298093.2019.1667131.

Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

• An H
• Jo IH
• Oh YL
• Jang KY
• Kong WS
• Sung JK
• So YS
• Chung JW

• KMID: 2470186
• DOI: http://doi.org/10.1080/12298093.2019.1667131

Abstract

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M(AF)) and the average number of alleles (N(A)) were 0.50 and 5.47, respectively. The average number of genotypes (N(G)), observed heterozygosity (H(O)), expected heterozygosity (H(E)), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M(AF) was negatively correlated with N(G) (−0.683), N(A) (−0.600), H(O) (−0.584), and PIC (−0.941). N(G), N(A), H(O), and PIC were positively correlated with other polymorphic parameters except for M(AF). UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.

Keyword

Button mushroom; cluster analysis; genetic diversity; simple sequence repeat marker

MeSH Terms

Agaricales*
Alleles
Breeding
Cluster Analysis
Gene Frequency
Genetic Variation*
Genotype
Microsatellite Repeats