Mycobiology.
2014 Jun;42(2):193-197.
Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer
- Affiliations
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- 1Department of Biomedical Chemistry, Konkuk University, Chungju 380-701, Korea. cslee@kku.ac.kr
- 2Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 440-706, Korea.
Abstract
- Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by P. ostreatus was 2.5% and 9 days, respectively. Reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. Our findings reveal that the use of apple pomace can be a model for the valuable addition of similar wastes and for the development of a solid-state fermenter and commercial production of oyster mushroom P. ostreatus.
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