Mycobiology.
2000 Sep;28(3):119-122.
Screening of Phytase Overproducing Strains in Aspergillus spp. by UV Mutagenesis
Abstract
- Phytases (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1.3.8) are enzymes which catalyze the hydrolisys of phytate into myo-inositol and inorganic phosphates. Phytases are found in plants and a variety of microorganisms. Aspergillus species were treated with 254 nm of UV irradiation for the screening of phytase overproducing mutant strains. At 15 minute irradiation, the survivals of population were less than 5%, and UV irradiation time was decided at 20 minute for the isolation of mutant strains. Four UV mutant strains in A. oryzae (YUV-47, -169, -341, -511) and six in A. ficuum (FUV-17, -36, -69, -193, -317, -419) were isolated on PSM media containing ammonium phosphate. The specific enzyme activities of A. ficuum mutants are 110 to 140% higher than that of wild type.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Culture Conditions and Characterizations of a New Phytase-Producing Fungal Isolate, Aspergillus sp. L117
- Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis
- Breeding and Screening of Lentinula edodes Strains Resistant to Trichoderma spp.
- Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa
- Reidentification of Aspergillus spp. Isolated from Clinical Specimens of Patients Suspected as Pulmonary Aspergillosis in Korea
