Mycobiology.  2009 Dec;37(4):267-271. 10.4489/MYCO.2009.37.4.267.

Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis

• Vu VH
• Pham TA
• Kim K

• KMID: 2053026
• DOI: http://doi.org/10.4489/MYCO.2009.37.4.267

Abstract

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of gamma-irradiation of Co60, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and beta-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.

Keyword

Aspergillus sp; Cellulase; Sequential repeated mutagenesis; Stability

MeSH Terms

Aspergillus
beta-Glucosidase
Cellulase
Mass Screening
Methylnitronitrosoguanidine
Mutagenesis
Sprains and Strains
Cellulase
Methylnitronitrosoguanidine
beta-Glucosidase