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Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis

Vu VH, Pham TA, Kim K

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential...
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Optimization of Fungal Enzyme Production by Trichoderma harzianum KUC1716 through Surfactant-Induced Morphological Changes

Lee H, Lee YM, Heo YM, Hong JH, Jang S, Ahn BJ, Lee SS, Kim JJ

The morphological optimization of Trichoderma harzianum was carried out using several surfactants to achieve increased cellulase production. Addition of the surfactants to the culture medium successfully modified the fungal morphology...
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Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation

Vu VH, Pham TA, Kim K

  • KMID: 2312606
  • Mycobiology.
  • 2011 Mar;39(1):20-25.
Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with Co60 gamma-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold...
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Enzyme Activity of Cenococcum geophilum Isolates on Enzyme-specific Solid Media

Obase K, Lee SY, Chun KW, Lee JK

  • KMID: 2312628
  • Mycobiology.
  • 2011 Jun;39(2):125-128.
Enzyme activities of Cenococcum geophilum isolates were examined on enzyme-specific solid media. Deoxyribonuclease, phosphatase, and urease were detected in all isolates, whereas cellulase was not detected in any of the...
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Effect of 'Azotobacter' Bioinoculant on the Growth and Substrate Utilization Potential of Pleurotus eous Seed Spawn

Eyini M, Parani K, Pothiraj C, Rajapandy

We investigated the effect of nitrogen fixing Azotobacter bioinoculant on the mycelial growth and the rate of substrate utilization by Pleurotus eous. The synergistic or antagonistic role of the microorganism...
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Evaluation of Ten Wild Nigerian Mushrooms for Amylase and Cellulase Activities

Jonathan S, Adeoyo OR

  • KMID: 2312623
  • Mycobiology.
  • 2011 Jun;39(2):103-108.
Amylases and cellulases are important enzymes that can be utilized for various biological activities. Ten different wild Nigerian mushrooms (Agaricus blazei, Agaricus sp., Corilopsis occidentalis, Coriolus versicolor, Termitomyces clypeatus, Termitomyces...
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Enzyme Activities and Substrate Degradation by Fungal Isolates on Cassava Waste During Solid State Fermentation

Pothiraj C, Eyini M

  • KMID: 2312530
  • Mycobiology.
  • 2007 Dec;35(4):196-204.
The growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. Rhizopus stolonifer showed the highest and the fastest utilization of starch...
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Laparoscopic Surgery for Removal of the Multiple Large Gastric Bezoars

Hong TH, Kim JJ, Park SM

  • KMID: 2372593
  • J Gastric Cancer.
  • 2010 Jun;10(2):84-86.
Bezoars are retained concretions of undigested animal or vegetable material that can produce gastrointestinal obstruction, ulceration, and bleeding. Therapeutic options for gastric bezoars include enzyme therapy (papain, cellulase, or acetylcysteine),...
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Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum

Jo WS, Park HN, Cho DH, Yoo YB, Park SC

  • KMID: 2312629
  • Mycobiology.
  • 2011 Jun;39(2):129-132.
To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the...
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Purification and Characterization of Intracellular Cellulase from Aspergillus oryzae ITCC-4857.01

Begum MF, Absar N

  • KMID: 2408782
  • Mycobiology.
  • 2009 Jun;37(2):121-127.
Purification and characterization of intracellular cellulase produced by A. oryzae ITCC-4857.01 are reported. The enzyme was purified by ion-exchange chromatography using DEAE-cellulose followed by Gel filtration. The purification achieved was...
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Comparison of Dyes for Easy Detection of Extracellular Cellulases in Fungi

Yoon JH, Park JE, Suh DY, Hong SB, Ko SJ, Kim SH

  • KMID: 2312507
  • Mycobiology.
  • 2007 Mar;35(1):21-24.
To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and...
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Detection of Extracellular Enzyme Activities in Ganoderma neo-japonicum

Jo WS, Park HN, Cho DH, Yoo YB, Park SC

  • KMID: 2312626
  • Mycobiology.
  • 2011 Jun;39(2):118-120.
The ability of Ganoderma to produce extracellular enzymes, including beta-glucosidase, cellulase, avicelase, pectinase, xylanase, protease, amylase, and ligninase was tested in chromogenic media. beta-glucosidase showed the highest activity, among the...
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Isolation and Analysis of the Enzymatic Properties of Thermophilic Fungi from Compost

Lee H, Lee YM, Jang Y, Lee S, Lee H, Ahn BJ, Kim GH, Kim JJ

  • KMID: 2312742
  • Mycobiology.
  • 2014 Jun;42(2):181-184.
To the best of our knowledge, this is the first report on thermophilic fungi isolated in Korea. Three species of thermophiles were isolated from compost and were identified as Myriococcum...
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Detection of Extracellular enzymes Activities in Various Fusarium spp.

Kwon HW, Yoon JH, Kim SH, Hong SB, Cheon Y, Ko SJ

Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested...
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Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum

Lee S, Park MS, Lee H, Kim JJ, Eimes JA, Lim YW

Agarum clathratum, a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying...
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Bioconversion of Lignocellulose Materials

Pothiraj C, Kanmani P, Balaji P

  • KMID: 2312489
  • Mycobiology.
  • 2006 Dec;34(4):159-165.
One of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. Phanerochaete chrysosporium is one of the important commercially cultivated fungi...
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New Record of Mariannaea elegans var. elegans in Korea

Tang L, Hyun MW, Yun YH, Suh DY, Kim SH, Sung GH

  • KMID: 2408207
  • Mycobiology.
  • 2012 Mar;40(1):14-19.
A Mariannaea fungus was isolated during investigation of an elm tree infested with unidentified beetles. Based on morphological characteristics and molecular analysis of the internal transcribed spacer rDNA sequence, the...
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Isolation, Regeneration and PEG-Induced Fusion of Protoplasts of Pleurotus pulmonarius and Pleurotus florida

Eyini M, Rajkumar K, Balaji P

  • KMID: 2312482
  • Mycobiology.
  • 2006 Jun;34(2):73-78.
Inter-specific hybridization between Pleurotus pulmonarius and P. florida was attempted through PEG-induced protoplast fusion to select a fusant. The protocol for protoplast release, regeneration and fusion in these two Pleurotus...
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Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

Lim SH, Lee YH, Kang HW

  • KMID: 2312705
  • Mycobiology.
  • 2013 Dec;41(4):214-220.
This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost...
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Biocontrol of Fusarium Crown and Root Rot and Promotion of Growth of Tomato by Paenibacillus Strains Isolated from Soil

Xu SJ, Kim BS

  • KMID: 2312739
  • Mycobiology.
  • 2014 Jun;42(2):158-166.
In this study, bacterial strains were isolated from soils from 30 locations of Samcheok, Gangwon province. Of the isolated strains, seven showed potential plant growth promoting and antagonistic activities. Based...
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