Mycobiology.  2013 Sep;41(3):159-163. 10.5941/MYCO.2013.41.3.159.

Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa

• Kim SR
• Kang HW
• Ro HS

• KMID: 1964231
• DOI: http://doi.org/10.5941/MYCO.2013.41.3.159

Abstract

A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and beta-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in beta-glucan productivity by producing 0.254 and 0.236 mg soluble beta-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble beta-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.

Keyword

Beta-glucan; Chemical mutagenesis; Growth rate; Sparassis crispa

MeSH Terms

Agaricales
Efficiency
Mass Screening
Mesylates
Methyl Methanesulfonate
Mutagenesis
Sprains and Strains
Mesylates
Methyl Methanesulfonate