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Growth Characteristics of Polyporales Mushrooms for the Mycelial Mat Formation

Bae B, Kim M, Kim S, Ro HS

Mushroom strains of Polyporales from the genera Coriolus, Trametes, Pycnoporus, Ganoderma, and Formitella were explored in terms of mycelial growth characteristics for the application of mushroom mycelia as alternative sources...
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Analysis of Genetic Diversity and Population Structure of Wild Strains and Cultivars Using Genomic SSR Markers in Lentinula edodes

Lee HY, Moon S, Ro HS, Chung JW, Ryu H

In this study, the genetic diversity and the population structure of 77 wild strains and 23 cultivars of Lentinula edodes from Korea were analyzed using 20 genomic SSRs, and their...
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Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones

Ha B, Kim S, Kim M, Ro HS

Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction...
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Nucleus-Selective Expression of Laccase Genes in the Dikaryotic Strain of Lentinula edodes

Ha B, Lee S, Kim S, Kim M, Moon YJ, Song Y, Ro HS

In mating of Lentinula edodes, dikaryotic strains generated from certain monokaryotic strains such as the B2 used in this study tend to show better quality of fruiting bodies regardless of...
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A Rare Case of Pulmonary Epithelioid Hemangioendothelioma Presenting with Skin Metastasis

Ro HS, Shin JY, Roh SG, Lee NH, Yang KM, Moon WS

Epithelioid hemangioendothelioma (EHE) is a well-differentiated and rare vascular tumor. Systemic metastases are uncommon. Herein, we present a patient with skin metastasis of pulmonary EHE (PEH) that was treated by...
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Differential Expression of Laccase Genes in Pleurotus ostreatus and Biochemical Characterization of Laccase Isozymes Produced in Pichia pastoris

Park M, Kim M, Kim S, Ha B, Ro HS

In this study, transcriptome analysis of twelve laccase genes in Pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. Lacc5 and Lacc12 were specifically expressed in...
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Isolation and Characterization of Monokaryotic Strains of Lentinula edodes Showing Higher Fruiting Rate and Better Fruiting Body Production

Ha BS, Kim S, Ro HS

The effects of monokaryotic strains on fruiting body formation of Lentinula edodes were examined through mating and cultivation of the mated dikaryotic mycelia in sawdust medium. To accomplish this, monokaryotic...
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Current Technologies and Related Issues for Mushroom Transformation

Kim S, Ha BS, Ro HS

Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign...
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Cloning and Molecular Characterization of beta-1,3-Glucan Synthase from Sparassis crispa

Yang YH, Kang HW, Ro HS

  • KMID: 2312740
  • Mycobiology.
  • 2014 Jun;42(2):167-173.
A beta-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble beta-1,3-glucan (beta-glucan). Sequencing and subsequent open reading...
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Isolation of a Variant Strain of Pleurotus eryngii and the Development of Specific DNA Markers to Identify the Variant Strain

Lee HJ, Kim SW, Ryu JS, Lee CY, Ro HS

  • KMID: 2312722
  • Mycobiology.
  • 2014 Mar;42(1):46-51.
A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of...
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Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa

Kim SR, Kang HW, Ro HS

A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S....
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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

Kim SW, Kim S, Lee HJ, Park JW, Ro HS

  • KMID: 2312712
  • Mycobiology.
  • 2013 Dec;41(4):252-255.
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four...
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Breeding of New Strains of Mushroom by Basidiospore Chemical Mutagenesis

Lee J, Kang HW, Kim SW, Lee CY, Ro HS

  • KMID: 2312653
  • Mycobiology.
  • 2011 Dec;39(4):272-277.
Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia...
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Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains

Shi S, Ro HS

  • KMID: 2312568
  • Mycobiology.
  • 2010 Mar;38(1):70-73.
Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the...
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Determination of Mineral Components in the Cultivation Substrates of Edible Mushrooms and Their Uptake into Fruiting Bodies

Lee CY, Park JE, Kim BB, Kim SM, Ro HS

  • KMID: 2408780
  • Mycobiology.
  • 2009 Jun;37(2):109-113.
The mineral contents of the cultivation substrates, fruiting bodies of the mushrooms, and the postharvest cultivation substrates were determined in cultivated edible mushrooms Pleurotus eryngii, Flammulina velutipes, and Hypsizigus marmoreus....
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Retrotransposon Microsatellite Amplified Polymorphism Strain Fingerprinting Markers Applicable to Various Mushroom Species

Le QV, Won HK, Lee TS, Lee CY, Lee HS, Ro HS

The retrotransposon marY1 is a gypsy family retroelement, which is detected ubiquitously within the fungal taxonomic groups in which mushrooms are included. To utilize marY1 as a molecular marker for...
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Isolation of Bacteria Associated with the King Oyster Mushroom, Pleurotus eryngii

Lim Y, Ryu JS, Shi S, Noh W, Kim E, Le QV, Lee HS, Ro HS

Eight distinct bacteria were isolated form diseased mycelia of the edible mushroom, Pleurotus eryngii. 16S rDNA sequence analysis showed that the isolates belonged to a variety of bacterial genera including...
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MushBase: A Mushroom Information Database Application

Le VQ, Lee HS, Ro HS

A database application, namely MushBase, has been built based on Microsoft Access in order to store and manage different kinds of data about mushroom biological information of species, strains and...
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Culture Conditions Affecting the Optimal Mycelial Growth of Cystoderma amianthinum

Shim SM, Oh YH, Lee KR, Kim SH, Im KH, Kim JW, Lee UY, Shim JO, Shim MJ, Lee MW, Ro HS, Lee HS, Lee TS

Cystoderma amianthinum, one of edible fungi belongs to Agaricaceae of Basidiomycota, has a good taste and flavor. This study was carried out to obtain the basic informations for the optimum...
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The Characteristics of Cultural Conditions for the Mycelial Growth of Macrolepiota procera

Shim SM, Oh YH, Lee KR, Kim SH, Im KH, Kim JW, Lee UY, Shim JO, Shim MJ, Lee MW, Ro HS, Lee HS, Lee TS

Macrolepiota procera, one of edible mushrooms belongs to Agaricaceae of Basidiomycota, has a good taste and good medicinal value. As a preliminary study for the development of artificial cultivation method...
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