J Korean Acad Periodontol.  2001 Mar;31(1):179-191. 10.5051/jkape.2001.31.1.179.

Effect of Bisphosphonate on Osteoblastic Activity of the Human Periodontal Ligament Cells in Vitro

• Kim EY
• Kim OS
• Chung HJ

• KMID: 2045144
• DOI: http://doi.org/10.5051/jkape.2001.31.1.179

Abstract

Previous studies have demonstrated an increase in bone mass and density with use of bisphosphonate in osteoporosis. This agent acts as an inhibitor of osteoclastic activity and results in increase of net osteoblastic activity. The purpose of the present study was to examine the effect of the bisphosphonate on osteoblastic activity of the human periodontal ligament cells in vitro. Periodontal ligament cells were primarily obtained from extracted healthy third molars. Cells of 4th to 6th passage were cultured in Dulbecco's modified Eagle's medium containing alendronate sodium or etidronate disodium at the concentration of 10(-12)~10(-6) mol/L in 5% CO2 incubator at 37degrees C. Cell count and MTT assay for cellular activity were done at 2 to 7 days of culture. Alkaline phosphatase activity at 4 to 7 days of culture and formation of mineralized nodules at 28 days of culture with addition of 50 microgram/ml ascorbic acid, 10 mM beta-glycerophosphate , 10(-7) M dexamethasone were evaluated. 1. Alendronate sodium Compared to the control, the proliferation of periodontal ligament cells was generally increased and the cellular activity was maintained at 2 days of culture and generally decreased at 7 days of culture. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. 2. Etidronate disodium The proliferation of periodontal ligament cells was increased at 2 days of culture and decreased or maintained at 7 days of culture. Compared to the control, the cellular activity of periodontal ligament cells was generally decreased. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. These results suggest that alendronate sodium and etidronate disodium may have a potential effect on osteoblastic lineage of periodontal ligament cells, distinct from their inhibitory action on osteoclasts and could contribute to enhance periodontal regeneration and alveolar bone regeneration.

MeSH Terms

Alendronate
Alkaline Phosphatase
Ascorbic Acid
Bone Regeneration
Cell Count
Dexamethasone
Etidronic Acid
Humans*
Incubators
Molar, Third
Osteoblasts*
Osteoclasts
Osteoporosis
Periodontal Ligament*
Regeneration
Alendronate
Alkaline Phosphatase
Ascorbic Acid
Dexamethasone
Etidronic Acid