Korean J Dermatol.  1993 Apr;31(2):157-162.

Laminin B1 mRNA expression in squamous cell carcinoma of the skin

• Lee KS
• Rho YJ
• Song JY

Abstract

BACKGROUND: Laminin, which is present in all basement membrane, is a multifunctional glycoprotein with diverse biological activities. Like fibronectin, it can influence cell adhesion, growth, morphology, differentiation, and migration as well as the assembly of the extracellular matrix. Laminin primarily affects cells of epithelial origin, and response varies depending on the cells.
OBJECTIVE
Studies about the relationship of laminin and the pathogenesis or metastasis of squamous cell carcinoma(SCC). MEHTODS: In this study, we have examined the laminin B1 mRNA expression and localizing pattern of laminin in SCC, using Northern and slot-blot hybridization, in situ hybridization and immunoperoxidase stain.
RESULTS
In Northern blot hybridization, 5.6Kb mRNA transcript was detected in control and SCC specimens. Slot-blot hybridization indicated theat amounts of laminin B1 mRNA expression in SCC was 677.0+/-268.5 densitometer absorbance unit, in control was 228.0+/-16.37 unit respectively. There is a 3-fold increase in SCC compared to control. In in situ hybridization, 35S-labeled laminin B1 cDNA-mRNA hybrid granules were detected along the basement membrane and adjacent cells of SCC. In immunoperoxidase stain, laminin has stained strongly and continuously along the basement membrane of the tumor nest in well differentiated SCC, and weakly and interrupted in poorly differentiated SCC.
CONCLUSION
These results suggested that laminin might be related to the pathogenesisand metastasis of squamous cell carcinomas.

MeSH Terms

Basement Membrane
Blotting, Northern
Carcinoma, Squamous Cell*
Cell Adhesion
Extracellular Matrix
Fibronectins
Glycoproteins
In Situ Hybridization
Laminin*
Neoplasm Metastasis
RNA, Messenger*
Skin*
Fibronectins
Glycoproteins
Laminin
RNA, Messenger