Korean J Dermatol.  2000 Mar;38(3):352-357.

Expression of Dermal Keratinocyte Growth Factor mRNA in Cutaneous Squamous Cell Carcinoma and Basal Cell Carcinoma

• Cun TJ
• Whang KK
• Hong CK
• Ro BI
• Seo SJ

Abstract

BACKGROUND: Human keratinocyte growth factor(KGF) is an epithelial cell specific mitogen which is secreted by normal stromal fibroblast. In skin, KGF has been shown to stimulate keratinocyte proliferation.
OBJECTIVE
In the present study, we sought to determine whether KGF is expressed in human squamous and basal cell carcinoma.
METHODS
Using a reverse transcriptase polymerase chain reaction(RT-PCR), we examined the expression of KGF mRNA in normal and cancerous human skin tissues.
RESULTS
1. The 583 bp KGF specific PCR product was observed in normal human cultured fibroblast, but no PCR product was seen in normal human cultured keratinocyte, A431, PAM212, HaCaT, and XB2 cell lines by RT-PCR of KGF mRNA using KGF-specific primer as visualized on an ethidium bromide stained agarose gel. 2. The 583 bp KGF specific PCR product was observed in the normal human dermis, squamous and basal cell carcinoma samples. 3. All three squamous and basal cell carcinoma samples revealed significant overexpression of KGF mRNA transcript in comparison with normal skin tissue.
CONCLUSION
These results suggest that KGF mRNA level may be elevated in squamous and basal cell carcinoma and elevated KGF may enhance cellular proliferation of these skin carcinoma.

Keyword

Keratinocyte growth factor mRNA; Squamous cell carcinoma; Basal cell carcinoma

MeSH Terms

Carcinoma, Basal Cell*
Carcinoma, Squamous Cell*
Cell Line
Cell Proliferation
Dermis
Epithelial Cells
Ethidium
Fibroblast Growth Factor 7*
Fibroblasts
Humans
Keratinocytes*
Polymerase Chain Reaction
RNA, Messenger*
RNA-Directed DNA Polymerase
Sepharose
Skin
Ethidium
Fibroblast Growth Factor 7
RNA, Messenger
RNA-Directed DNA Polymerase
Sepharose