J Korean Cancer Assoc.  2000 Aug;32(4):801-809.

Correlation between Genetic Polymorphism of CYP2D6 and CYP1A1 and Susceptibility of Renal Cell Carcinoma in Korean

• Park KW
• Jung SI
• Jung GW
• Kwon HY
• Jeong JS
• Chun JH
• Yoon JH

Abstract

PURPOSE: Many of the enzymes handling environmental factors are polymorphic and may confer variable susceptibility to renal cell carcinoma (RCC). Among those, the author studied genetic polymorphisms of CYP2D6 (B & T) and CYP1A1 in RCCs and controls in Korean.
MATERIALS AND METHODS
Using 132 RCCs and 94 controls, first PCR products were obtained in 104 RCCs and 94 controls with CYP2D6, and 74 RCCs and 56 controls with CYP1A1. Res triction enzyme - BstN I/EcoN I for CYP2D6 (B & T), and NCo I for CYP1A1-digestion was followed to analyze constitutive DNA.
RESULTS
In both RCCs and controls, no mutant allele of CYP2D6 (B & T) was detected and the susceptibility for occurrence of RCC was unable to evaluate. With CYP1A1 RFLP, homozy gous wild type (WW) was seen in 68 (52.3%; 37 RCCs, 31 controls), heterozygous mutant type (WM) in 54 (41.5%; 32 RCCs, 22 controls) and homozygous mutant type (MM) in 8 (6.2%; 5 RCCs, 3 controls). The odds ratios (95% CI) of RCC susceptibility for CYP1A1 genotype were 1.15 for WM and 1.36 for MM. Even though not significant statistically, higher tendency in MM presented.
CONCLUSION
There is no association between susceptibility for the occurrence of RCC and genetic polymorphism of CYP2D6 (B & T) and CYP1A1.

Keyword

Renal cell carcinoma; CYP2D6; CYP1A1; Genetic polymorphism

MeSH Terms

Alleles
Carcinoma, Renal Cell*
Cytochrome P-450 CYP1A1*
Cytochrome P-450 CYP2D6*
DNA
Genotype
Odds Ratio
Polymerase Chain Reaction
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Cytochrome P-450 CYP1A1
Cytochrome P-450 CYP2D6
DNA