Korean J Prev Med.  1998 Mar;31(1):1-14.

PCR and RFLP-based CYP2D6(B) and CYP2D6(T) Genotyping for Korean Lung Cancer Cases and Controls

• Chun JH
• Lee CH
• Urm SH
• Son BC
• Park JH
• Jung KO
• Sohn CH
• Yoon HK
• Son CH
• Kim HI
• Kim JS

Abstract

The genetically determined CYP2D6 activity is considered to be associated with cancer susceptibility with inter-individual variation. Genetic polymorphism of CYP2D6(B) and CYP2D6(T) was determined by the two polymerase chain reaction(PCR) and BstN1 and EcoN1 restriction fragment length polymorphisms(RFLP) for 67 lung cancer cases and 95 healthy volunteer controls. The cases were composed of 26 squamous cell carcinoma, 14 small cell carcinoma, 10 adenocarcinoma, 3 large cell undifferentiated carcinoma, and 14 not histologically diagnosed. The results were gained from the 142 subjects (57 cases and 85 controls) who observed successfully in two PCR and BstN1/EcoN1 RFLP. Only one and no mutant allele of the CYP2D6(B) and CYP2D6(T) gene was detected, that is, the frequency of mutant allele was very low; 0.7%(1/142) and 0%(0/142), respectively. Detected mutant allele of the CYP2D6(B) was heterozygous type(WM). The odds ratios for lung cancer susceptibility with CYP2D6(B) and CYP2D6(T) genotype were not calculated. These results are similar to the previous understanding that the mutant allele is very rare in Orientals compared to Caucasians, therefore, it considered that CYP2D6(B) and CYP2D6(T) genotypes have maybe no association with lung cancer susceptibility in Koreans. This is the basic data of CYP2D6(B) and CYP2D6(T) genotypes for Koreans. It would be hepful for further study to determine lung cancer susceptibility of Koreans with the data about CYP1A1, CYP2E1, GSTM1 from future study.

Keyword

Genetic polymorphism; CYP2D6(B); CYP2D6(T); lung cancer susceptibility

MeSH Terms

Adenocarcinoma
Alleles
Carcinoma
Carcinoma, Small Cell
Carcinoma, Squamous Cell
Cytochrome P-450 CYP1A1
Cytochrome P-450 CYP2D6
Cytochrome P-450 CYP2E1
Genotype
Healthy Volunteers
Lung Neoplasms*
Lung*
Odds Ratio
Polymerase Chain Reaction*
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Cytochrome P-450 CYP1A1
Cytochrome P-450 CYP2D6
Cytochrome P-450 CYP2E1