J Korean Acad Conserv Dent.  2005 Jan;30(1):1-6. 10.5395/JKACD.2005.30.1.001.

Effects of Enterococcus faecalis sonicated extracts on IL-2, IL-4 and TGF-beta1 production from human lymphocytes

• Kim HS
• Jang SW
• Shon WJ
• Lee ST
• Kim CH
• Lee WC
• Lim SS

• KMID: 1986990
• DOI: http://doi.org/10.5395/JKACD.2005.30.1.001

Abstract

In order to examine the immunoresponse of host cells to Enterococcus faecalis, this in vitro study monitored the production of Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Transforming growth factor-beta1 (TGF-beta1) in human lymphocytes. Lymphocytes were activated with PHA in the presence or abscence of sonicated extracts of E. Faecalis (SEF) and further incubated for 72 hours. The level of each cytokine was measured by ELISA. Data were analyzed with Kruskal-Wallis test and Mann-Whitney U test (P < 0.05). PHA-activated group did exhibit higher level of IL-2 and IL-4 than untreated control group. The levels of expression of both cytokines were significantly decreased following the treatment of high (25 microg/ml) and medium concentration (12.5 microg/ml) of SEF (P <0 .05) than those of PHA activated group. But low concentration (5 microg/ml) of SEF showed the similar level of IL-2 and IL-4 production as those of PHA activated group. TGF-beta1 was unaffected by SEF treatment. These results suggested that E. faecalis may suppress IL-2 and IL-4 production by lymphocytes and this could be one of possible factors why E. faecalis are found frequently in the teeth with failed endodontic treatment.

Keyword

Enterococus faecalis; Lymphocyte; IL-2; Il-4; TGF-beta1; Sonicated extract

MeSH Terms

Cytokines
Enterococcus faecalis*
Enterococcus*
Enzyme-Linked Immunosorbent Assay
Humans*
Interleukin-2*
Interleukin-4*
Lymphocytes*
Tooth
Transforming Growth Factor beta1*
Cytokines
Interleukin-2
Interleukin-4
Transforming Growth Factor beta1

Figure

• 

  Figure 1 Response to SEF (Sonicated extract of E. faecalis) of IL-2, IL-4 and TGF-β1 production in the supernatants of lymphocytes. The cell was treated with PHA and various concentrations of each SEF for 72 hr. The data represent mean values (n = 6 duplicate culture for each group).

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