J Korean Acad Conserv Dent.  2005 Mar;30(2):138-144. 10.5395/JKACD.2005.30.2.138.

Effect of sonicated extracts of Enterococcus faecalis on the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils

• Park SK
• Shon WJ
• Lim SS

• KMID: 1987006
• DOI: http://doi.org/10.5395/JKACD.2005.30.2.138

Abstract

This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5mg/ml) for 7 days, and lipopolysaccharides (LPS) of E. coli. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test. In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05microg/ml) of SEF (p < 0.05), but it decreased with an increase in the concentration of SEF (p < 0.05). In the case of SEF treated with Ca(OH)2, all of the MMP levels were higher than negative control group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05). All of the levels in E. coli LPS were increased with increasing concentrations (p < 0.05). According to this study we could summarize as follows: 1. MMP-8 was expressed at low level in untreated PMN group and the levels of MMP-8 were upregulated in PMN stimulated by E. coli LPS groups. 2. In the SEF groups, the level of production of MMP-8 decreased with an increase in the concentration of SEF (p < 0.05). So E. faecalis may have suppressive effect on the production of MMP-8 by PMN. 3. In the case of SEF treated with Ca(OH)2, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p < 0.05), but no statistical difference was found among the different SEF concentrations (p > 0.05).

Keyword

E. faecalis; MMP-8; Calcium hydroxide; PMN leukocyte

MeSH Terms

Calcium Hydroxide
Enterococcus faecalis*
Enterococcus*
Enzyme-Linked Immunosorbent Assay
Humans*
Lipopolysaccharides
Matrix Metalloproteinase 8*
Neutrophils*
Calcium Hydroxide
Lipopolysaccharides
Matrix Metalloproteinase 8

Figure

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  Figure 1 Average concentration of MMP-8 (± SD) (ng/mol)

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  Figure 2 Average concentration of MMP-8 (ng/mol)

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