Korean J Gynecol Oncol.  2008 Mar;19(1):57-67. 10.3802/kjgo.2008.19.1.57.

Expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 as prognostic factors in endometrial cancer

• Choi JR
• Lee TH
• Oh YL
• Lee CJ
• Kim WG

• KMID: 1979502
• DOI: http://doi.org/10.3802/kjgo.2008.19.1.57

Abstract

OBJECTIVE
Homeostasis of the extracellular matrix (ECM) is maintained by the action of a specific system of proteolytic enzymes known as matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP). The MMP/TIMP system regulates the composition and turnover of ECM to control the site and extent of connective tissue remodeling. In pathologic conditions, MMP play a key role in degradation of basement membrane and extracellular matrix, and is responsible for cancer invasion, progression and metastasis. The aim of this study is to evaluate the correlation between expressions of MMP/TIMP and clinicopathologic factors in endometrial cancer.
METHODS
Expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 were assessed by immunohistochemistry in a total of 55 endometrial cancers and were analyzed by the correlation between expressions of MMP/TIMP and clinicopathologic factors in endometrial cancer.
RESULTS
Expression rates of MMP-2,-9, TIMP-1, and TIMP-2 were 71.7%, 54.9%, 41.2%, and 76.5% respectively. Expression of MMP-2 was correlated with the group of positive lymph node metastasis in endometrial cancer (p=0.04). Specially, coexpression of MMP-2 and TIMP-2 was significantly more frequent in the group of positive lymph node metastasis (p<0.01) and the group of positive peritoneal
CONCLUSION
The expressions of MMP and TIMP were not a significant difference in survival analysis, but this study was recognized that the coexpression MMP-2 and TIMP-2 is correlated with lymph node metastasis and positive peritoneal cytology.

Keyword

Endometrial cancer; Matrix metalloproteinase; Tissue inhibitors of metalloproteinase; Prognostic factor

MeSH Terms

Basement Membrane
Connective Tissue
Endometrial Neoplasms
Extracellular Matrix
Female
Homeostasis
Immunohistochemistry
Lymph Nodes
Matrix Metalloproteinases
Metalloproteases
Neoplasm Metastasis
Peptide Hydrolases
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinases
Metalloproteases
Peptide Hydrolases
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2

Figure

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  Fig. 1 Isolated endometrial tumor glands show focal immunostaining to MMP-2 in tumor cell cytoplasm (×200, MMP-2).

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  Fig. 2 Endometrial tumor glands show diffuse immunostaining to MMP-9 in tumor cell cytoplasm and stromal cell components (×200, MMP-9).

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  Fig. 3 Isolated endometrial tumor glands show focal immunostaining to MMP-2 in tumor cell cytoplasm (×200, MMP-2).

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  Fig. 4 Endometrial tumor glands show diffuse immunostaining to TIMP-2 in tumor cell (×200, TIMP-2).

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