Korean J Asthma Allergy Clin Immunol.  2012 Jun;32(2):115-121.

Anti-inflammatory Effect of Green Tea Cell Water in Activated Raw 264.7 Cells with Lipopolysaccharide

Abstract

BACKGROUND
The biochemical properties of green tea extracts can generally be divided into 4 aspects: antioxidative, anticarcinogenic, anti-inflammatory and antiradiation activities. Green tea extracts have 20-fold more antioxidative activity than vitamin C and also have a wide range of anti-inflammatory activity. Therefore, it is presumed that it would play a role in the treatment of chronic inflammatory disorders. Pathophysiology of immunologic disorders involves overexpression of proinflammatory mediators, including nitric oxide (NO) and prostaglandin E2 (PGE2).
METHODS
After the treatment of different concentrations for Green Tea Cell Water in lipopolysaccharide (LPS)-treated Raw 264.7 cells, the levels of NO and PGE2 were measured in the media. Furthermore, we analyzed the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and phosphorylation of inhibitory kappa Balpha using western blot.
RESULTS
Green Tea Cell Water suppressed the LPS-induced expression of iNOS and COX-2 in a concentration-dependent manner. Phosphorylation of the inhibitory-kappaB was also inhibited.
CONCLUSION
Our results suggest that Green Tea Cell Water may be a significant inflammatory factor and can be used a therapeutic modality in managing chronic inflammatory diseases.

MeSH Terms

Ascorbic Acid
Cyclooxygenase 2
Dinoprostone
Nitric Oxide
Nitric Oxide Synthase
Phosphorylation
Prostaglandins
Tea
Water
Ascorbic Acid
Cyclooxygenase 2
Dinoprostone
Nitric Oxide
Nitric Oxide Synthase
Prostaglandins
Tea
Water