Korean J Pediatr.
2006 Feb;49(2):198-202. 10.3345/kjp.2006.49.2.198.
Fas/FasL expression in the hippocampus of neonatal rat brains follwing hypoxic-ischemic injury
- Affiliations
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- 1Department of Pediatrics, College of Medicine, Dankook University, Cheonan, Korea. ychang@dankook.ac.kr
- 2Department of Anatomy, College of Medicine, Dankook University, Cheonan, Korea.
- 3Institute of Life Science, College of Medicine, Dankook University, Cheonan, Korea.
- 4Department of Biological Science, Dankook University, Cheonan, Korea.
- KMID: 1966614
- DOI: http://doi.org/10.3345/kjp.2006.49.2.198
Abstract
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PURPOSE: Fas is a cell surface receptor that transduces apoptotic death signals. Interaction of extracelluar domain of Fas with Fas ligand(FasL) triggers the apoptotic process in many diseases. We investigated the expression of Fas and FasL in the hippocampus of 7-day-old newborn rat brains following hypoxia-ischemia injury.
METHODS
The 7-days-old newborn rats were exposed to 8 percent oxygen for two hours after the ligation of right common carotid arteries. The newborn rats were killed and their brains were removed at 12, 14 and 48 hours after hypoxic-ischemic injury. The expressions of Fas and FasL of the right hippocampus were observed by western blotting and immunofluorescent staining.
RESULTS
Fas and FasL were strongly expressed in the right hippocampus ipsilateral to the ligation of the common carotid artery by western blotting at 12 hours following hypoxic-ischemic injury, and then slowly decreased. The immunofluorescent expressions of Fas and FasL strongly increased in the CA1 area of the right hippocampus at 12 and 24 hours following hypoxic-ischemic injury. The immunofluorescent expression of Fas decreased at 48 hours, but the expression of FasL persisted strongly at 48 hours following hypoxic-ischemic injury.
CONCLUSION
The interaction of Fas with FasL on the cell surface may be involved in neuronal injury following hypoxic-ischemic injury in the developing brain.
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