Korean J Fertil Steril.  1998 Dec;25(3):287-292.

The Cytoskeletal and Chromosomal Constitution in Vitrified Mouse Oocyte

Abstract

SUMMARY: The objective of this study was to confirm whether the vitrification method using EFS35 has detrimental effect for cytoskeleton and chromosome constitution of the mouse oocytes by indirect immunocytochemistry and chromosome analysis. Mouse oocytes were vitrified using EFS35 which consisted of 35% ethylene glycol, 18% ficoll, 0.3 M sucrose and 10% FBS in M2 medium. The results obtained in this experiment were summarized as follows: When the survival rates after being exposed or vitrified in EFS35 were examined, there were not different between two groups (97.7 and 89.3%). Also, when the microtubule morphology and microfilament distribution in vitrified oocytes were examined, normal percentage of two cytoskeleton in vitrified group (95.5 and 100%) was not different from that in control (97.5 and 100%) and exposed group (92.3 and 100%). In addition, the rate of oocytes containing a normal chromosome number in vitrified group (73.5%) after IVF was not different from that in control (79.5%) and exposed group (78.7%). These results indicated that the cytoskeletal morphology and chromosome constitution of mouse oocytes were not affected by cryoprotectant (EFS35) or freezing and that vitrification methods using EFS35 was suitable for cryopreservation of mouse oocytes.


MeSH Terms

Actin Cytoskeleton
Animals
Constitution and Bylaws*
Cryopreservation
Cytoskeleton
Ethylene Glycol
Ficoll
Freezing
Immunohistochemistry
Mice*
Microtubules
Oocytes*
Sucrose
Survival Rate
Vitrification
Ethylene Glycol
Ficoll
Sucrose
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